在GC-MS上进行尿液有机酸液体液体提取方法的小型化和自动化协议
Masauso Moses Phiri1,2, Elmarie Davoren2, Barend Christiaan Vorster2
1Department of Pathology and Microbiology, School of Medicine, University of Zambia, Lusaka 10101, Zambia.
Molecules (Basel, Switzerland)
|August 12, 2023
概括
这项研究成功地缩小和自动化了尿道有机酸提取,提高了效率和准确性. 这种新方法可以减少溶剂的使用,提高诊断测试的可靠性.
科学领域:
- 临床化学 临床化学
- 分析化学 分析化学
- 生物化学 生物化学
背景情况:
- 手动提取尿中的有机酸是耗时的,容易导致结果变化.
- 目前的方法缺乏效率和标准化,影响诊断的准确性.
研究的目的:
- 为了缩小和自动化尿道有机酸的内部提取方法.
- 提高效率,减少错误,提高有机酸分析的可靠性.
主要方法:
- 重新评估和缩小了现有的内部提取协议,减少了步骤和体积.
- 使用汉密尔顿微实验室Star液体处理器自动化了小型化方法.
- 用来自ERNDIM外部质量保证计划的样本验证了自动化方法.
主要成果:
- 成功缩小和自动化尿道有机酸提取.
- 已建立的线性范围 (0-300 mg/L) 和高相关系数 (r >0.99) 对13种分析物.
- 在大多数有机酸的变化系数 (CVs) 低于20%的情况下,已证明可接受的精度.
结论:
- 自动提取方法与手动的内部方法相当或优于.
- 自动化减少了溶剂消耗,增加了样品吞吐量,并最大限度地减少了诊断中的错误.
- 这种方法在常规临床实验室应用中具有重大潜力.
更多相关视频
09:38Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures
Published on: January 7, 2019
8.6K
08:41Integration of Miniaturized Solid Phase Extraction and LC-MS/MS Detection of 3-Nitrotyrosine in Human Urine for Clinical Applications
Published on: July 14, 2017
9.4K
相关概念视频
Gas Chromatography–Mass Spectrometry (GC–MS)
4.4K
Gas chromatography–mass spectrometry (GC–MS) is the combination of analytical techniques of gas chromatography and mass spectrometry in a single instrument for analyzing a mixture of compounds. The gas chromatograph separates the compounds in the mixture, and the mass spectrometer analyzes each compound separately to determine the molecular masses and molecular structures.
A gas chromatograph consists of a long, narrow capillary column with a polysiloxane coating on the inner wall....
A gas chromatograph consists of a long, narrow capillary column with a polysiloxane coating on the inner wall....
4.4K
Mass Spectrometry: Complex Analysis
825
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
825
