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甘eIF4E基因与抵抗甘条马赛克病毒之间的关系

Hongli Shan¹, Du Chen¹, Rongyue Zhang¹

¹Sugarcane Research Institute, Yunnan Academy of Agricultural Science, Yunnan Key Laboratory of Sugarcane Genetic Improvement, Kaiyuan 661699, China.

Plants (Basel, Switzerland)

|August 12, 2023

概括

由于真核转化启动因子4E (eIF4E) 基因的固有差异,甘串状马赛克病毒 (SCSMV) 的耐药性因品种而异. 在eIF4E中的特定突变

科学领域:

植物病理学植物病理学
分子遗传学分子遗传学
农业科学农业科学

背景情况:

甘马赛克病是由甘条马赛克病毒 (SCSMV) 引起的,严重影响了甘的产量和质量.
细胞转化启动因子4E (eIF4E) 作为植物中的天然耐药基因,与蛋白质突变相关的衰退性耐药性.

研究的目的:

调查甘品种CP94-1100和ROC22之间的SCSMV耐药性差异的遗传基础.
分析eIF4E基因变异在甘中产生马赛克抗性的作用.

主要方法:

对抗性和易受性甘品种中eIF4E基因序列的比较分析.
在eIF4E编码区域内识别单核酸多态 (SNP) 和插入/删除 (InDel) 位点.
预测eIF4E蛋白的空间结构,并识别突变部位.

主要成果:

在eIF4E开放读取框架 (ORF) 中的四基误解突变导致了多种保存域,与SCSMV电阻相关.
eIF4E编码区域显示了28个SNP位点和没有InDel位点的保护,受到负面选择的影响.
在耐药 (Yuetang 55) 和易感 (ROC22) 甘中发现了eIF4E盖口袋中的特定非同义氨基酸突变,这表明它在SCSMV耐药性中发挥了作用.

结论:

甘对SCSMV耐药性的差异归因于它们eIF4E基因的固有变异.
在eIF4E蛋白结构中确定突变位可能对调节甘中马赛克耐药性至关重要.
这些发现为进一步研究eIF4E在甘疾病耐药性的功能机制提供了基础.

关键词:

氨基酸变化的氨基酸变化细胞转化启动因子4E (eIF4E)不同名的突变非同名的突变电阻差异的不同.甘串状马赛克病毒 (SCSMV) 是一种

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