在Strychnos spinosa Lam.中评估遗传变异 使用简单序列重复标记器的形态型.
Zoliswa Mbhele1, Godfrey Elijah Zharare2, Clemence Zimudzi3
1Department of Botany, Faculty of Science, Agriculture and Engineering, University of Zululand, KwaDlangezwa 3886, South Africa.
Plants (Basel, Switzerland)
|August 12, 2023
概括
这项研究使用简单序列重复 (SSR) 标记揭示了绿色子 (Strychnos spinosa) 的遗传多样性. 结果显示了不同的形态型和三个亚种群,有助于未来的作物改进.
科学领域:
- * 植物学 植物学
- * 遗传学 在遗传学方面
- * 分子生物学 * 分子生物学
背景情况:
- * 绿子 (Strychnos spinosa) 是一种有价值的南非土著水果树,具有化潜力.
- * 对于Strychnos spinosa形态类型的分子多样性数据有限.
- * 了解遗传变异对于保护和作物改进至关重要.
研究的目的:
- * 为了确定32个斯特里克诺斯·斯皮诺萨形态型之间的遗传变异.
- * 开发和利用简单序列重复 (SSR) 标记器用于分子特征.
- * 为了研究Strychnos spinosa的种群结构.
主要方法:
- *在32种斯特里克诺斯旋形态型中放大了14种简单序列重复 (SSR) 标记.
- *对等位基因数,多态信息含量 (PIC) 和遗传距离的分析.
- * 用贝叶斯推理来分析人口结构.
主要成果:
- * 总共确定了159个等位基因,平均每位基因为5.68.
- *多态信息内容 (PIC) 值在0.22到0.84.8之间.
- * 形态类型基于遗传距离聚集在一起,主要与周围骨纹理相关.
- *使用贝叶斯分析确定了三个不同的子群体,与遗传距离保持一致.
结论:
- *这是第一个报告SSR标志物开发和应用Strychnos spinosa遗传多样性和种群结构的研究.
- * 分子特征提供了关于Strychnos spinosa的遗传景观的见解.
- *研究结果支持Strychnos spinosa的有针对性的育种和作物改进计划,可能提高粮食安全.
相关概念视频
Single Nucleotide Polymorphisms-SNPs
15.3K
A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
15.3K
Genetic Variation
323
Genetic variation is the diversity in DNA sequences found among individuals of the same species. This diversity is crucial for a species' survival because it helps organisms adapt to environmental changes. Genetic variation begins with fertilization, where an egg and sperm cell merge. Each of these cells carries 23 chromosomes, up to 46 in the fertilized egg. Chromosomes are long DNA strands that contain genes, the basic units of heredity.
Genes exist in different versions called alleles,...
Genes exist in different versions called alleles,...
323
Evolutionary Relationships through Genome Comparisons
5.8K
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
5.8K
Comparing Copy Number Variations and SNPs
17.7K
Sequencing of the human genome has opened up several best-kept secrets of the genome. Scientists have identified thousands of genome variations that exist within a population. These variations can be a single nucleotide or a larger chromosomal variation.
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
17.7K
Trihybrid Crosses
23.5K
Trihybrid Crosses
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal...
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal...
23.5K
Mutation, Gene Flow, and Genetic Drift
58.5K
In a population that is not at Hardy-Weinberg equilibrium, the frequency of alleles changes over time. Therefore, any deviations from the five conditions of Hardy-Weinberg equilibrium can alter the genetic variation of a given population. Conditions that change the genetic variability of a population include mutations, natural selection, non-random mating, gene flow, and genetic drift (small population size).
58.5K


