基于牛血红蛋白的氧载体-201 (Hemopure) 在四个血液学分析仪上的干扰
Marinke W Bronkhorst-van der Helm1, Floor Weerkamp2, Albert Huisman3
1Department of Clinical Chemistry, Erasmus MC, University Medical Center, Rotterdam, The Netherlands.
International journal of laboratory hematology
|August 12, 2023
概括
像Hemopure这样的基于血红蛋白的氧气载体会干扰标准的血红蛋白实验室测试. 流细胞测量方法准确地测量细胞内血红蛋白,通过计算总和细胞血红蛋白之间的差异来实现治疗监测.
科学领域:
- 临床化学 临床化学
- 血液学 血液学 血液学
- 生物医学工程 生物医学工程
背景情况:
- 基于血红蛋白的氧气载体 (HBOCs),如HBOC-201 (Hemopure),被开发用于在输血不可或拒绝时治疗急性贫血.
- HBOC-201在血中自由循环,可能会干扰标准实验室诊断测试.
- 准确的实验室监测对于接受HBOC治疗的患者的管理至关重要.
研究的目的:
- 评估HBOC-201对常用的血液学分析仪的干扰.
- 确定可靠的方法来监测患者的HBOC-201治疗.
主要方法:
- 血液样本中添加了治疗性HBOC-201剂量.
- 使用四种不同的自动分析仪 (Sysmex XN-20,西门子Advia 2120i,Abbott Alinity Hq,Abbot Cell Dyn Sapphire) 分析了九个血液学参数.
- 结果与对照样本进行比较,以确定偏差和干扰.
主要成果:
- 大多数血液学参数,包括细胞计数,血红素和MCV,显示出最小的偏差.
- 标准总血红蛋白 (Hb) 测量,MCH和MCHC受到HBOC-201干扰的显著影响.
- 基于流细胞计的Hb测量准确量化了细胞内Hb,不包括HBOC-201.
结论:
- 总Hb,MCH和MCHC测量在存在HBOC-201时是不可靠的.
- 流细胞计提供了一种准确的方法来测量细胞内Hb.
- 总Hb和细胞Hb之间的差异量化HBOC-201度,使治疗监测成为可能.
相关概念视频
Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences
516
Inductively coupled plasma–mass spectrometry (ICP–MS) is a highly selective and sensitive technique for accurate elemental analysis. Though the analysis of ICP–MS mass spectra is comparatively straightforward, it is affected by spectroscopic and non-spectroscopic interferences. Spectroscopic interferences arise when the plasma contains ionic species with an m/z value the same as the analyte ion. Spectroscopic interference can be categorized as isobaric, polyatomic ions, and...
516
Atomic Absorption Spectroscopy: Interference
831
Interference leads to systematic error in atomic absorption (AA) measurements by enhancing or diminishing the analytical signal or the background. These interferences can be grouped into three main categories: spectral interference, chemical interference, and physical interference.
Spectral interference occurs when signals from other elements or molecules overlap with the analyte signal, falsely elevating or masking the analyte's absorbance. This interference can be corrected using Zeeman,...
Spectral interference occurs when signals from other elements or molecules overlap with the analyte signal, falsely elevating or masking the analyte's absorbance. This interference can be corrected using Zeeman,...
831
Blood Transfusion and Agglutination
10.9K
Blood transfusion is a therapeutic measure to restore the blood volume after extensive blood loss due to an accident or a medical procedure. Blood transfusion involves drawing a certain amount of blood from a suitable donor and infusing it into the recipient.
History
The history of blood transfusion dates back to the 17th century, when early attempts were made in animals. In 1818 James Blundell, a British doctor, performed the first successful human blood transfusion. Later in 1900, Karl...
History
The history of blood transfusion dates back to the 17th century, when early attempts were made in animals. In 1818 James Blundell, a British doctor, performed the first successful human blood transfusion. Later in 1900, Karl...
10.9K
Blood Transfusion
613
Blood transfusion is a critical medical procedure that saves lives and treats various medical conditions. It involves transferring blood from a donor to a recipient. This process requires a thorough understanding of the ABO blood group system and its associated antigens and antibodies.
Blood Transfusion Overview
A blood transfusion is a medical procedure used to replace blood lost due to injury, surgery, or to treat conditions such as anemia or cancer. During a transfusion, donor blood is...
Blood Transfusion Overview
A blood transfusion is a medical procedure used to replace blood lost due to injury, surgery, or to treat conditions such as anemia or cancer. During a transfusion, donor blood is...
613
Oxygen Transport in the Blood
2.8K
Hemoglobin (Hb) is a crucial molecule in the human body, consisting of four polypeptide chains, each bound to an iron-containing heme group. This unique structure enables hemoglobin to bind to oxygen, with each molecule capable of combining with four molecules of oxygen, leading to rapid and reversible oxygen loading. When fully loaded with oxygen, it is called oxyhemoglobin, while hemoglobin that has released oxygen is called reduced hemoglobin or deoxyhemoglobin. As hemoglobin binds oxygen,...
2.8K
Hemoglobin
3.7K
Hemoglobin is a globular protein made up of four subunits. Two of these subunits are alpha chains, and the other two are beta chains. Each subunit contains a molecule of heme, which has an iron atom and can bind to oxygen. When an oxygen molecule binds to one heme group, it changes the shape of hemoglobin, making it easier for the other heme groups to bind oxygen as well.
When all four heme groups are bound to oxygen, the resulting molecule is called oxyhemoglobin. As a result, arterial blood...
When all four heme groups are bound to oxygen, the resulting molecule is called oxyhemoglobin. As a result, arterial blood...
3.7K


