脂肪细胞Na,K-ATPase信号减弱了实验性尿素性心肌病
Komal Sodhi1, Xiaoliang Wang2, Muhammad A Chaudhary3
1Departments of Medicine, Surgery, and Biomedical Sciences, Joan C. Edwards School of Medicine, Marshall University, Huntington, WV, USA. sodhi@marshall.edu.
Cellular and molecular biology (Noisy-le-Grand, France)
|August 12, 2023
概括
向脂肪细胞Na,K-ATPase信号改善了小鼠的尿路性心肌病症和相关的氧化应激. 这突显了脂肪细胞功能障碍在慢性病进展中的关键作用.
科学领域:
- 心血管生物学 心血管生物学
- 代谢疾病 代谢疾病
- 腎臟醫學 腎臟醫學
背景情况:
- 氧化应激和脂肪细胞内细胞内信号的改变有助于肥胖并发症和心血管问题.
- -腺三酸盐酶 (Na,K-ATPase) 信号的失调与全身炎症和代谢障碍有关.
- 细胞Na,K-ATPase信号传递在尿路性心肌病中的作用需要进一步调查.
研究的目的:
- 调查脂肪细胞Na,K-ATPase信号传递在减轻实验性尿素性心肌病变的特定作用.
- 为了确定是否向脂肪细胞Na,K-ATPase信号可以在小鼠模型中预防或逆转心脏和代谢变化.
主要方法:
- 在小鼠中通过部分切除术 (PNx) 诱导实验性尿路性心肌病变.
- 通过使用带有adiponectin促进剂的晶状病毒,实现了Na,K-ATPase抑制剂NaKtide的细胞特异性表达.
- 疾病表型,脂肪细胞分子变化,全身炎症和氧化应激被评估,有或没有西方饮食.
主要成果:
- 部分切除术诱导心肌病,贫血,脂肪细胞功能障碍,炎症和氧化应激在4周内.
- 这些病态变化被西方饮食加剧了.
- 脂肪细胞特异性NaKtide表达显著预防或改善了这些PNx诱导的变化,而骨肌肉特异性表达没有.
- 脂肪细胞功能障碍和尿路性心肌病因脂肪细胞特异性NaKtide表达而显著改善.
结论:
- 脂肪细胞Na,K-ATPase信号传递在尿路性心肌病的发展和进展中起着关键作用.
- 脂肪细胞内的氧化环境在部分切除术的背景下对驱动尿路性心肌病症至关重要.
- 向脂肪细胞Na,K-ATPase信号传递代表了慢性功能衰竭并发症的潜在治疗策略.
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