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通过向Lin28a,miR-188-5p沉默可以改善大脑缺血/再输液损伤

Dan Hou¹, Chaoying Pei¹, Dan Yu²

¹Department of Neurology, Haikou Affiliated Hospital of Central South University Xiangya School of Medicine, Haikou, Hainan, 570208, China.

Metabolic brain disease

|August 12, 2023

概括

通过减少神经元亡和炎症,MicroRNA-188-5p沉默可以防止大脑缺血/反 (I/R) 损伤. 这通过其对Lin28a的调节而发生,为缺血性中风提供了一个新的治疗点.

科学领域:

神经科学是一个神经科学.
分子生物学分子生物学
生物化学生化学

背景情况:

大脑缺血/反 (I/R) 损伤涉及复杂的病理调节网络.
微RNAs (miRNAs) 在调节细胞过程中起着至关重要的作用,包括细胞亡和炎症.

研究的目的:

研究miR-188-5p在脑I/R损伤的病理调节网络中的作用.
在此背景下阐明miR-188-5p和Lin28a之间的监管关系.

主要方法:

使用MACO/R和OGD/R治疗方法建立了脑I/R损伤模型.
评估了神经元活力,细胞亡,蛋白质和细胞因子水平以及miRNA/mRNA表达.
证实了miR-188-5p和Lin28a之间的相互作用,使用Lin28a沉默来进一步探索调节机制.

主要成果:

在I/R模型中,miR-188-5p是上调的,而Lin28a是下调的.
沉默miR-188-5p减少了心脏病发作量,改善了神经行为学分数,并减少了脑.
miR-188-5p沉默增强了神经元活力,减少了亡,调节了与亡相关的蛋白质 (Bax,Bcl-2,分裂的caspase-3) 和炎症性细胞因子 (TNF-α,IL-1β,IL-6).
miR-188-5p对Lin28a的表达进行了负调节,而额外的Lin28a沉默则逆转了保护作用.

结论:

miR-188-5p沉默通过调解Lin28a表达来缓解神经元亡和脑I/R损伤中的炎症.
miR-188-5p和Lin28a之间的相互作用为缺血性中风提供了一个新的治疗策略.

关键词:

缺血/再输液损伤缺血/再输液损伤缺血性中风是因为缺血性中风.林28a 在线28a 在 miR-188-5p 中.

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