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使用色度分割G四重复探针检测多重复的NASBARNA产品

Maria S Rubel1, Liubov A Shkodenko2, Daria A Gorbenko2

  • 1Laboratory of DNA-Nanosensor Diagnostics, ITMO University, Saint Petersburg, Russia. rubel@scamt-itmo.ru.

Methods in molecular biology (Clifton, N.J.)
|August 12, 2023
PubMed
概括

这项研究介绍了用于检测RNA片的G-四重复DNA传感器. 这些传感器提供了色度信号,简化了对具有挑战性的结构RNA目标的识别.

关键词:
折叠的RNA是一种折叠RNA.基于核酸序列的放大 (NASBA)基于过氧酶的DNA酶分开 (二进制) 探头.视觉检测 视觉检测是一种视觉检测.

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Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
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