核酸纳米粒子 (NANPs) 的抗凝活性通过完全自动化系统的血栓生成动力学来评估
Renata de Freitas Saito1, Bárbara Gomes Barion2, Tania Rubia Flores da Rocha2
1Comprehensive Center for Precision Oncology, Centro de Investigação Translacional em Oncologia (LIM24), Departamento de Radiologia e Oncologia, Faculdade de Medicina da Universidade de São Paulo and Instituto do Câncer do Estado de São Paulo, São Paulo, SP, Brazil. renata.saito@hc.fm.usp.br.
Methods in molecular biology (Clifton, N.J.)
|August 12, 2023
概括
快速可逆的抗凝剂,如基于寡核酸的药物,可以通过补充DNA/RNA序列去活化. 这种方法可以快速恢复正常的血液凝固,提供显著的临床潜力.
科学领域:
- 生物化学 生物化学
- 药理学 药理学是指药理学的学科.
- 分子生物学分子生物学
背景情况:
- 快速可逆的抗凝剂对于临床应用至关重要.
- 基于寡核酸的抗凝剂提供了一种独特的方法,通过补充序列去活化.
- 脏过有效地去除由治疗性和抗药性寡核酸形成的非活性双重体.
研究的目的:
- 探索快速可逆抗凝剂的临床潜力.
- 描述使用血栓生成 (TG) 的基于寡核酸的抗凝剂的评估.
- 详细说明校准自动血栓计 (CAT) 试验的使用,以评估抗凝剂的疗效.
主要方法:
- 测量血栓生成 (TG) 以评估抗凝能力.
- 使用DNA/RNA血结合的aptamer携带纤维作为一个例子.
- 使用完全自动化的校准自动血栓谱 (CAT) 试验.
主要成果:
- 在CAT测定检测TG通过测量光信号从一个灭的基板.
- 光信号被转换成TG曲线来量化抗凝剂活性.
- 不活跃的双重体的形成有效地恢复了正常的凝血.
结论:
- 基于寡聚核酸的抗凝剂可以通过反补寡聚物迅速被禁用.
- 血栓生成试验,特别是自动化CAT试验,对于评估这些新型抗凝剂是有效的.
- 这种方法对开发安全和可控制的抗凝固疗法具有前景.
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