在anammox细菌内部,细胞内碳依赖DNRA的电子转移机制
1Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education, China), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China.
Water research
|August 12, 2023
概括
亚纳莫克斯细菌可以使用细胞内碳去除酸盐,实现95%的总去除. 这项研究阐明了用于增强污染物去除的电子转移机制.
科学领域:
- 环境微生物学 环境微生物学
- 生物化学 生物化学
- 废水处理 废水处理
背景情况:
- 无氧氨氧化 (anammox) 将氨和化物转化为气,但产生酸盐.
- 亚纳莫克斯细菌可以通过使用细胞内碳的异型酸盐降解为氨 (DNRA) 来降解酸盐.
- 亚纳莫克斯细菌中细胞内碳依赖DNRA的电子转移机制尚不清楚.
研究的目的:
- 为了阐明anammox细菌中细胞内碳依赖DNRA的电子转移机制.
- 为了确定单独的anammox细菌是否可以超过理论上的89%的总去除效率.
- 评估将DNRA与anammox结合以提高去除的可行性.
主要方法:
- 转录组分析以确定关键的酶和途径.
- 代谢抑制剂实验探测电子转移路径.
- 光现场杂交 (FISH) 和定量PCR (qPCR) 用于评估细菌活力和繁殖.
主要成果:
- 来自细胞内葡萄糖原分解的NADH为酸盐转化提供电子.
- 电子通过ubiquinone和复合物III转移到酸盐减少酶 (Nar) 和酸盐减少酶 (NrfA).
- 一种联合的anammox和细胞内碳依赖的DNRA工艺在测序批量反应堆中实现了95%的总去除.
结论:
- 在anammox细菌中,细胞内碳依赖DNRA的电子转移途径已被阐明.
- 单独的阿纳莫克斯细菌可以通过整合DNRA获得高的总去除效率 (>89%) .
- 这项研究增强了对anammox细菌在去除污染物的代谢能力的理解.
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