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线下二维LC-tandemMS的menaquinones来自热友细菌的线下二维LC

Elizaveta Timkina¹, Irena Jarošová Kolouchová¹, Lucie Kyselová²

¹Department of Biotechnology, University of Chemistry and Technology, Technická 5, 166 28 Prague, Czech Republic.

|August 12, 2023

概括

热友细菌产生各种不同的menaquinone (MK) 同类物. Geobacillus kaustophilus被确定为一个关键菌株,产生MKs与5-15异烯单元和只有转变异构体,至关重要的生物活性.

科学领域:

微生物学微生物学
生物化学生物化学
生物技术是生物技术.

背景情况:

梅纳基诺 (MKs) 是一种基本化合物,其生物活性基于异烯单元和双键配置而变化.
热友细菌通常会产生不同于常见菌株的MK同类,例如Bacillus subtilis.
了解热友生物的MK多样性对于生物技术应用至关重要.

研究的目的:

为了识别产生广泛的MK同类菌株的细菌菌株.
确保所选择的菌株只能产生MKs.的全转体几何同体.
描述热友细菌的MK生产特征.

主要方法:

使用了离线二维液态染色学-并联质谱法 (LC-MS/MS).
采用RP18和COSMOSIL的胆固醇分离阶段,以双键几何学为基础.
在不同温度下培养热友细菌以分析MK生产.

主要成果:

Geobacillus kaustophilus被确定为一种产生广泛的MK同类物种的菌株.
这项研究证实,G. kaustophilus只能产生全跨几何同体.
从MK-5 (五个异烯基单元) 到MK-15 (十五个异烯基单元) 的menaquinones被确定.

结论:

盖奥巴西勒斯 (Geobacillus kaustophilus) 是一种具有生物技术意义的菌株,可以生产特定的menaquinone同类物.
该菌株仅生产全转化异构体,简化了下游应用.
能够生产5至15个异烯单元的MK,为各种生物应用提供了潜力.

关键词:

这两种Cis/trans同位素的同位素.LC/ESI在 MS 的同时.这些是menaquinones.射门枪质谱仪质谱仪.热友细菌是一种热友细菌.

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