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重组猪流行性腹病毒NSP9的表达和免疫性

Shiguo Liu¹, Qijia Yu¹, Sha Li¹

¹Institute of Pathogenic Microorganism and College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang, Jiangxi, China; Nanchang Key Laboratory of Animal Virus and Genetic Engineering, Jiangxi Agricultural University, Nanchang, 330045, China.

|August 12, 2023

概括

猪流行性腹病毒 (PEDV) 感染导致小猪的严重损失. 这项研究表明,PEDV非结构蛋白9 (Nsp9) 是免疫的,可以用来开发有效的PEDV疫苗.

科学领域:

病毒学病毒学
免疫学免疫学免疫学
兽医医学兽医医学兽医医学

背景情况:

猪流行性腹病毒 (PEDV) 由于新生小猪的高死亡率,在养猪业造成重大经济损失.
PEDV是一种RNA病毒,它依赖其非结构蛋白9 (Nsp9) 来进行复制,使Nsp9成为抗病毒干预的潜在目标.

研究的目的:

为了研究PEDV非结构蛋白9 (Nsp9) 的免疫性.
评估NSP9作为开发抗PEDV疫苗和抗病毒药物的目标的潜力.

主要方法:

在大肠杆菌中表达和净化了PEDV Nsp9蛋白,与MBP和His标签融合.
通过比较幽默和细胞免疫反应来评估MBP-Nsp9和His-Nsp9的免疫性.
猪用NSP9蛋白免疫,以评估其刺激免疫反应和产生中和抗体的能力.

主要成果:

与His-Nsp9.9相比,使用MBP-Nsp9的免疫引发了更强的幽默和细胞免疫反应.
用NSP9蛋白免疫猪成功刺激了猪的免疫系统,引发了幽默性免疫反应.
针对NSP9产生的抗体在体外 (Vero细胞) 证明了抑制PEDV增殖的能力.

结论:

PEDV Nsp9蛋白具有免疫性,可以刺激猪的幽默性和细胞免疫力.
对Nsp9的抗体的发展可以抑制PEDV复制,支持其作为疫苗候选人的潜力.
这些发现为开发新型疫苗和用于PEDV预防的抗病毒策略提供了基础.

关键词:

免疫性免疫性免疫性在 MBP MBP MBP 在 Nsp9 中使用.在PEDV中,您可以使用PEDV.

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