臭氧通过触发由H2O2和铁引起的氧化细胞死亡来调解空气等离子体的抗癌作用
Manami Suzuki-Karasaki1, Yushi Ochiai2, Shizuka Innami2
1Department of Research and Development, Plasma ChemiBio Laboratory, Nasushiobara, Tochigi, Japan; Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan.
European journal of cell biology
|August 12, 2023
概括
血处理溶液中的臭氧 (O3) 通过引起线粒体损伤和氧化应激,选择性地杀死癌细胞. 这种由过氧化和铁介导的效果,模仿了空气等离子体激活介质的作用.
科学领域:
- 生物化学 生物化学
- 细胞生物学 细胞生物学
- 血医学是一种血医学.
背景情况:
- 冷大气等离子体和等离子体处理的溶液显示出瘤选择性癌症治疗的前景.
- 氧化应激是关键的,但具体的化学介质仍然不清楚.
- 之前的研究确定了癌细胞中的空气等离子体激活介质 (APAM) 和单极周核线粒体集群 (MPMC).
研究的目的:
- 调查臭氧 (O3) 在APAM抗癌作用中的作用.
- 确定O3是否是APAM瘤选择性细胞毒性的关键调解者.
- 阐明O3诱导癌细胞死亡的机制.
主要方法:
- 创建了一个没有氧化的臭氧化介质 (ODM).
- 评估了ODM对癌症和非恶性细胞的细胞毒性.
- 测量MPMC,基,脂类过氧化物和治疗细胞中的细胞内铁水平.
- 利用催化酶和铁化来研究保护作用.
主要成果:
- ODM 显示出强大的瘤选择性细胞毒性.
- ODM诱导了MPMC,增加了基激素和脂质过氧化物,并改变了癌细胞中的铁分布.
- catalase和铁化抑制了ODM诱导的细胞毒性和MPMC.
- 臭氧,而不仅仅是过氧化,对MPMC和细胞毒性至关重要.
结论:
- 臭氧是APAM瘤选择性抗癌作用的关键媒介.
- 臭氧通过过氧化和铁触发癌细胞中的氧化细胞死亡.
- 这些发现支持O3作为基于血的癌症疗法的关键组成部分.
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