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LcERF10 作为利奇果切割的积极调节剂

Zidi He¹, Xingshuai Ma¹, Fei Wang¹

¹State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China; Guangdong Litchi Engineering Research Center, College of Horticulture, South China Agricultural University, Guangzhou 510642, China.

International journal of biological macromolecules

|August 12, 2023

概括

乙烯信号调节了植物器官的流失. 研究人员发现LcERF10,一个关键的转录因子,通过抑制LcNHX7表达和改变脱离区域的pH,促进了利奇果子脱离.

科学领域:

植物生物学植物生物学
分子遗传学分子遗传学
园艺园艺园艺园艺

背景情况:

乙烯是一种关键的植物激素,调节植物器官脱离.
乙烯诱导脱离的精确分子机制尚未完全理解.
了解这些机制对于控制利奇等作物中的水果下降至关重要.

研究的目的:

为了确定涉及乙烯诱导的利奇果切割的关键调节基因.
为了阐明在脱离过程中识别的基因的分子功能.
调查LcERF10在调节果脱落区域内pH值变化的作用.

主要方法:

识别和表征素反应因子在.
基因沉默 (RNAi) 用于评估LcERF10在利奇果切割中的功能.
在果切割区 (FAZ) 中分析细胞溶液 pH 变化.
促进体结合试验和基因表达分析 (定量PCR).
在Arabidopsis中LcERF10的宫外表达,以研究保存的功能.

主要成果:

乙烯反应因子LCERF10被确定为利奇果切割的关键调节剂.
在乙烯激活切割过程中,FAZ显著诱导了LCERF10表达.
沉默LcERF10降低了果脱落,并削弱了FAZ中的细胞质化.
LcERF10直接抑制了Na+/H+交换器LcNHX7的表达.
在Arabidopsis中LcERF10的宫外表达促进了花器官脱离和细胞质化.

结论:

转录因子LcERF10在利奇果脱落中发挥着关键的积极作用.
LcERF10通过抑制LcNHX7和影响FAZ中的pH动态来调节乙烯的作用.
这项研究为植物激素调节的脱离的分子途径提供了新的见解.

关键词:

细胞溶液的pH值欧洲农业基金 (ERF) 是一个基金.果切割的结果是什么?子 (Litchi chinensis Sonn.) 是一种在中国生长的植物.纳+) / +) 交换器

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