来自印度孟买的一家社区诊所的女性的阴道微生物群变异的映射
Deepti Tandon1, Nainisha Shah1, Mayuri Goriwale1
1Department of Clinical Research, ICMR-National Institute for Research in Reproductive and Child Health, Mumbai, India.
Indian journal of medical microbiology
|August 12, 2023
概括
阴道微生物群,由乳杆菌主导,在非怀孕的妇女中差异很大. 无症状的细菌性阴道炎 (BV) 和最近的分娩强烈影响这些微生物社区的转变.
科学领域:
- 微生物学 微生物学
- 妇女健康 妇女健康
- 生殖健康 生殖健康
背景情况:
- 阴道微生物组在妇女的生殖健康中起着至关重要的作用.
- 微生物组成因生理和病理因素而波动.
- 了解这些变化是保持阴道健康的关键.
研究的目的:
- 为了绘制非孕妇的阴道微生物组.
- 根据影响因素评估阴道微生物组的变化.
- 评估无症状细菌性阴道炎 (BV) 和最近分娩对阴道环境的影响.
主要方法:
- 招募了52名性活跃,非怀孕的女性 (18-45岁).
- 使用Illumina HiSeq.收集了16SrRNA基因测序 (V3-V4区域) 的阴道拭子.
- 通过QIIME 2分析了微生物组数据;通过Nugent评分和细胞因子分析评估了阴道环境.
主要成果:
- 乳杆菌内菌是最丰富的物种 (34.3%).
- 无症状的BV显示了乳杆菌,Prevotella,Anaerococcus丰富度,IFN-γ表达和多样性的显著变化.
- 最近的分娩 (<12个月) 与乳杆菌,双菌,形菌的丰度变化以及较高的异常Nugent分数相关.
结论:
- 在这个孟买队列中,乳杆菌内体是占主导地位的阴道物种.
- 在无症状的BV和分娩后观察到显著的阴道微生物组干扰,特别是乳杆菌主导地位的丧失.
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