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铜反应调节器CsoR间接地参与了Bradyrhizobium diazoefficiens的脱
Pedro J Pacheco1, Juan J Cabrera1, Andrea Jiménez-Leiva1
1Department of Soil and Plant Microbiology, Estación Experimental del Zaidín, CSIC, C/Profesor Albareda 1, E-18008 Granada, Spain.
FEMS microbiology letters
|August 12, 2023
概括
在Bradyrhizobium diazoefficiens中,CsoR抑制器控制了脱基因. 铜限制降低了nirK和norCBQD的表达,CsoR间接调节这些基因并抑制copA.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 分子生物学分子生物学
背景情况:
- 布拉迪瑞佐比乌姆 (Bradyrhizobium diazoefficiens) 具有一个完整的脱路径,涉及Nap,Nirk,cNor和Nos.
- 铜对Nirk和Nos活动至关重要,并影响脱基因表达.
- 铜限制降低了关键的脱基因如nirK,norCBQD和nosRZDFYLX的下调.
研究的目的:
- 调查CsoR蛋白在调节脱基因表达中的作用,以应对铜的可用性.
- 了解CsoR,铜恒温和B. diazoefficiens中的脱之间的关系.
主要方法:
- 在Bradyrhizobium diazoefficiens中构建一个csoR删除突变.
- 在脱条件下对野生型和突变菌株的生长分析.
- 定量逆转录PCR (qRT-PCR) 用于在不同铜条件下分析nirK,norCBQD和copA基因表达.
主要成果:
- 删除csoR并没有影响在去化条件下的增长.
- 在限制铜的脱条件下,与野生型相比,csoR突变体中的nirK和norCBQD表达显著较低.
- 在csoR突变体中,copA表达显著增加,这表明CsoR抑制了copA.
- 在铜限制下,CsoR在调节nirK和norCBQD表达方面发挥着间接作用.
结论:
- 在B. diazoefficiens中,CsoR作为copA基因的抑制剂.
- 在限制铜的条件下,CsoR间接影响非化基因nirK和norCBQD的表达.
- 这些发现阐明了一种新的调节机制,该机制将大豆内共生体中的铜恒温与脱联系起来.
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