截然不同的四素序列使植物能够特别识别微生物衍生的细胞外囊泡
Jinyi Zhu1, Qian Qiao1, Yujing Sun1
1Department of Plant Pathology, Nanjing Agricultural University, 210095, Nanjing, China.
Nature communications
|August 12, 2023
概括
植物病原体在细胞外囊泡 (EVs) 中使用四氨酸蛋白来逃避免疫反应. 植物通过识别特定的蛋白质序列来区分病原体EV和自我EV,从而触发对微生物真核生物的防御机制.
科学领域:
- 植物与微生物的相互作用
- 分子植物病理学 分子植物病理学
- 蜂通讯 蜂通讯是通过蜂通讯进行的.
背景情况:
- 细胞外囊泡 (EVs) 在动物中调解细胞间的通信,并在植物微生物相互作用中发挥作用.
- EV介导的植物微生物相互作用的机制尚未完全理解.
研究的目的:
- 为了研究来自oomycete病原体Phytophthora sojae的EVs在植物免疫反应中的作用.
- 为了识别由植物识别的特定的病原体衍生分子.
主要方法:
- 对Phytophthora sojae EVs的蛋白质组分析.
- 在Nicotiana benthamiana和大豆 (Glycine max) 中进行功能性测试.
- 类似受体激酶 (RLK) 相互作用研究.
主要成果:
- 在P. sojae EVs中确定了四氨酸蛋白 (PsTET1,PsTET3).
- PsTET3的细胞外循环 (EC2) 被植物RLKs (NbSERK3a/b) 识别,从而触发免疫力.
- 病原体TET蛋白被识别,而植物TET蛋白则没有,这是由于C-终端序列分歧.
- PsTET1和PsTET3都对P. sojae的毒性至关重要.
结论:
- 植物可以根据TET蛋白序列变异区分自我和非自我EV.
- 这种识别机制允许植物对病原性真核生物产生积极的防御反应.
- 拉斯帕宁代表了植物病原体相互作用中的关键分子接口.
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