猪下呼吸道抗生素耐药性的表征
Yunyan Zhou1,2, Jingquan Li1, Fei Huang1
1National Key Laboratory of Swine Genetic Improvement and Germplasm Innovation, Jiangxi Agricultural University, Nanchang, 330045, China.
Nature communications
|August 12, 2023
概括
猪肺中的抗生素耐药性基因 (ARGs) 被确定,揭示了与危及人类和动物健康的移动遗传元素的联系. 一些ARG与猪肺部疾病的严重程度相关.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 兽医医学 兽医医学 兽医医学
背景情况:
- 呼吸道疾病对猪业和人类健康构成重大威胁.
- 猪下呼吸道微生物组中抗生素耐药性基因 (ARG) 的组成和分布在很大程度上是未知的.
- 猪中ARG,移动遗传元素 (MGE) 和肺部健康之间的关系需要进一步研究.
研究的目的:
- 描述猪下呼吸道微生物组的抗生素耐药性.
- 调查ARG与MGEs和猪肺部健康指标的关联.
- 为了提供第一个全面的ARG概况在这个特定的猪微生物组.
主要方法:
- 来自675只实验猪的745个元基因组的分析.
- 鉴定和描述1228个属于372个ARG的开放式阅读框架.
- 研究ARG-MGE链接复合体及其在其他微生物组 (猪肠,人类肺部) 中的存在.
主要成果:
- 鉴定了372个ARG,与与Tn916家族MGE相关的四环素抗性基因.
- 在猪肠道和人类肺部微生物组中观察到ARG和MGE (Tn916家族,tnpA) 之间的链接复合体,表明基因转移的潜力.
- 甲基蛋白细菌,特别是大肠杆菌,是主要的ARG载体.
- 发现了73个与猪肺病变严重程度有显著关联的ARG.
结论:
- 这项研究为猪下呼吸道微生物组中ARG概况提供了第一个全面的概述.
- 这些发现强调了MGEs在猪,人类和环境之间调解ARG转移的潜力.
- 特定的ARG与猪的肺部健康状况有关,表明它们作为疾病生物标志物的潜力.
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