临床前肌和带模型:超越3R (替换,减少和改进) 到5W1H (为什么,谁,什么,在哪里,什么时候,如何)
Dianne Little1, Peter C Amadio2, Hani A Awad3
1Department of Basic Medical Sciences, The Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana, USA.
概括
没有单一的动物模型可以完美地代表肌和带损伤. 研究人员必须根据特定的研究问题仔细选择模型,考虑每个模型的独特优势和临床前研究的局限性.
科学领域:
- 整形医学研究 整形医学研究
- 生物医学工程 生物医学工程
- 兽医科学 兽医科学 兽医科学
背景情况:
- 临床前动物模型对于研究肌和带损伤至关重要.
- 2022年骨科研究会肌部会议的重点是为这些模型制定指导方针.
- 现有的旋转手套撕裂,带损伤和肌损伤的模型有很大的不同.
研究的目的:
- 总结了专家对八种手术小动物和大型动物模型的观点,用于肌和带损伤.
- 建立研究中选择和使用临床前动物模型的指导原则.
- 确定挑战,并提出解决方案,将动物模型的发现转化为临床实践.
主要方法:
- 在2022年整形外科研究会肌部分会议上提出的专家意见的审查.
- 分析了八种手术小型和大型动物模型 (旋转手腕, flexor肌,ACL,阿基里斯肌).
- 应用"为什么,谁,什么,在哪里,什么时候,以及如何" (5W1H) 框架来评估每个模型.
主要成果:
- 对于所有肌和带病理,没有单一的"黄金标准"动物模型.
- 每个动物模型都有不同的优点和缺点.
- 模型的选择在很大程度上取决于具体的研究问题和病理学.
结论:
- 仔细考虑模型选择是必不可少的,承认局限性和适合研究问题.
- 建议提供明确的理由,详细报告动物模型,加强合作.
- 从动物模型发展到临床实践的清晰翻译途径对于该领域的进步至关重要.
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