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不兼容和兼容的根茎生物都居住在豆类根结的细胞间空间中

Shingo Hata¹, Risa Tsuda¹, Serina Kojima¹

¹Faculty of Agriculture, Ryukoku University, Otsu, Japan.

Plant signaling & behavior

|August 13, 2023

概括

同时存在的细菌,包括根茎菌,可以居住在豆类根结节中. 这项研究揭示了像Bradyrhizobium diazoefficiens和Bradyrhizobium sp.这样的细菌. SSBR45可以占据受感染的细胞和结节内的细胞间空间.

科学领域:

微生物学微生物学
植物科学植物科学
同生相互作用的交互.

背景情况:

豆类的根结体是根茎菌和其他细菌的宿主,但它们的确切位置尚不清楚.
了解结核中的细菌息地对于理解共生关系和植物健康至关重要.

研究的目的:

研究不同细菌在豆类根结核中的空间分布和息地.
为了确定非结节性细菌是否与共生根茎菌共同殖民结节.

主要方法:

细菌菌株Bradyrhizobium diazoefficiens USDA122和Bradyrhizobium sp. 的光蛋白标签 (DsRed, eGFP) 的使用方法这是SSBR45.5的标志.
同焦激光扫描显微镜 (低放大和高放大) 以可视化大豆结节内的细菌定位.
传输电子显微镜用于观察莲花日本结中的Mesorhizobium loti.

主要成果:

在感染细胞中发现了Bradyrhizobium diazoefficiens USDA122,而Bradyrhizobium sp. 在感染细胞中发现了Bradyrhizobium diazoefficiens USDA122. SSBR45 占据了细胞间空间.
在共注射时,USDA122和SSBR45都在结核的共生体和细胞质中观察到.
USDA122的混合种群占据了细胞间空间,而单个种群占据了受感染的细胞.
Mesorhizobium loti也在莲花的细胞间空间中被发现.

结论:

细菌,包括根茎菌,通常居住在豆类根结体内的细胞间空间,除了感染的细胞.
这种细胞间殖民似乎比以前认为的更为广泛的现象.
这些发现挑战了关于细菌在豆类结体内定位的传统观点.

关键词:

甘氨酸最大值 (Glycine max) 是一种莲花 japonicus 莲花 japonicus 莲花 japonicus 莲花 japonicus 莲花 japonicus 莲花日本它们的细胞系是apoplast.光是一种光.根茎植物 (Rhizobia) 是一种植物.一个根结的根结.

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