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Updated: Aug 13, 2026

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
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单个微粒细胞空间有限的数字量化策略 (SMSDQ) 用于在单个分子水平上计算微RNA

Yuanwen Liang1, Desheng Chen1, Honghong Wang1

  • 1Beijing Key Laboratory for Bioengineering and Sensing Technology, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, 30 Xueyuan Road, Haidian District, Beijing, 100083, China.

Biosensors & bioelectronics
|August 13, 2023
PubMed
概括

我们开发了一种使用微珠和黄金纳米粒子单分子量化微RNA (miRNA) 的新方法. 这种数字方法为生物样本中miRNA检测提供了超高的灵敏度和特异性.

关键词:
点击反应反应 点击反应反应.黄金纳米颗粒 (AuNP) 是一种局部化的表面等离子体共振 (LSPR)单细胞分析是一种单细胞分析.单个微粒球的空间限制.计数微RNAs 的数量.

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Small interfering RNAs (siRNA)02:30

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Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...