乳头顶部殖民者来自健康奶牛的细菌对抗导致乳腺炎的金黄色葡萄球菌生物膜
Paula Isaac1, Luciana Paola Bohl1, Cintia Mariana Romero2
1Instituto Multidisciplinario de Investigación y Transferencia Agroalimentaria y Biotecnológica (IMITAB), CONICET-UNVM, Villa María, Córdoba, Argentina; Instituto Académico Pedagógico de Ciencias Básicas y Aplicadas de la Universidad Nacional de Villa María (IAPCByA-UNVM), Villa María, Córdoba, Argentina.
Research in veterinary science
|August 13, 2023
概括
一种新型的细菌菌株H21及其外聚糖体Levon有效地对抗牛乳炎的关键因素金黄色葡萄球菌生物膜. 这一发现为预防和治疗乳牛持续性乳腺炎感染提供了一种有希望的非细胞毒性方法.
科学领域:
- 兽医微生物学 兽医微生物学
- 细菌病原体的产生
- 生物膜研究 生物膜研究
背景情况:
- 黄金葡萄球菌是牛乳炎的主要原因,导致全球牛奶产量大幅下降.
- 病原体形成生物膜的能力有助于持续的乳内感染和抗生素治疗的耐药性.
- 乳微生物群代表了针对乳腺炎的新策略的潜在来源.
研究的目的:
- 调查来自牛乳头顶部的共生细菌菌株的潜力,以控制金黄色葡萄球菌.
- 确定负责对抗S. aureus生物膜的特定细菌代谢物.
- 评估确定代谢物的安全性和有效性,以控制牛乳腺炎.
主要方法:
- 从哺乳期母牛的乳头顶部分离和识别一种Bacillus菌株 (H21).
- 在体外评估Bacillus sp. H21能够抑制黄金葡萄球菌生物膜的形成,并破坏成熟的生物膜.
- 对抗生物膜活性负责的外聚糖的鉴定和表征.
- 在牛细胞系上对外聚糖的细胞毒性测定.
主要成果:
- 巴西勒斯种类 (Bacillus sp.) 的存在 H21对黄金葡萄球菌生物膜的形成和成熟的生物膜表现出显著的对抗作用,而不会影响细菌的活力.
- 由Bacillus产生的外聚糖体Levano被确定为关键的抗生物膜剂.
- 在测试条件下,Levan有效地消除了金黄色葡萄球菌生物膜.
- 莱万在有效度下对牛细胞系没有细胞毒性作用.
结论:
- 像H21菌株这样的共生菌种可以有效地抵消Staphylococcus aureus殖民和与牛乳腺炎相关的生物膜形成.
- 莱万是一种Bacillus exopolysaccharide,是一种有前途的,无毒的药物,可以控制黄菌生物膜.
- 这些发现支持开发利用Bacillus及其代谢物用于预防和治疗牛乳腺炎的新生物技术战略.
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