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来自丝状真菌Acremonium chrysogenum的一个 (+) -cubenene合成酶的识别

Chang Chen¹, Ge Yao¹, Fuli Wang¹

¹State Key Laboratory of NBC Protection for Civilian, Beijing, 102205, People's Republic of China.

Biochemical and biophysical research communications

|August 13, 2023

概括

研究人员发现了一种来自Acremonium chrysogenum的新型 (+) -cubenene合成酶,AcTPS3. 在Saccharomyces cerevisiae的代谢工程使得 (+) -cubenene的高水平生物合成成为可能,实现了创纪录的标位.

科学领域:

生物化学生物化学
分子生物学分子生物学
代谢工程是代谢工程.

背景情况:

甲合成酶是生产甲的关键酶,这是各种工业中有价值的化合物.
许多真菌塞斯基特合成酶仍然没有表征,限制了它们的工业应用.

研究的目的:

为了识别和生化表征一种来自Acremonium chrysogenum的新型甲合成酶.
为了设计一个微生物宿主,以高效地生产基产品.

主要方法:

序列分析和体外酶试验被用于识别AcTPS3.
用Saccharomyces cerevisiae的代谢工程来实现异质蛋白表达和产品过度生产.
气色谱-质谱学 (GC-MS) 和核磁共振 (NMR) 谱学被用于产品的识别和量化.

主要成果:

鉴定出ActTPS3是一种四烯合成酶.
确定AcTPS3的主要产品是 (+) -cubenene (55.46%).
一种经过工程改造的Saccharomyces cerevisiae菌株获得了597.3mg/L的 (+) -cubenene生物合成标位,这是迄今为止报告的最高标位.

结论:

证实AcTPS3是第一个报告的 (+) -cubenene合成酶.
代谢工程策略可以显著增强微生物生产有价值的甲,如 (+) -cubenene.

关键词:

(+) - 基基烯的使用情况这就是Acremonium chrysogenum.生物合成生物合成赛斯基二烯合成酶是什么

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