相关实验视频

Updated: Jul 19, 2025

Quantitative, Real-time Analysis of Base Excision Repair Activity in Cell Lysates Utilizing Lesion-specific Molecular Beacons
15:01

Quantitative, Real-time Analysis of Base Excision Repair Activity in Cell Lysates Utilizing Lesion-specific Molecular Beacons

Published on: August 6, 2012

13.7K

在FLARE定量PCR的定义DNA序列中检测氧化修饰基损伤

Lang Pan1, Yaoyao Xue1, Ke Wang1

  • 1Departments of Microbiology and Immunology, School of Medicine, University of Texas Medical Branch at Galveston, Galveston, TX, USA.

Methods in molecular biology (Clifton, N.J.)
|August 13, 2023
PubMed
概括

这项研究引入了一种新的方法,FLARE qPCR,用于检测常见的DNA损伤8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo(d) Gua. 这种技术可以在来自哺乳动物细胞的小DNA样本中识别这种重要的DNA损伤标记物.

关键词:
造成DNA基损伤.在FLARE合的rt-qPCRR中,基因调节序列的基因调节序列

更多相关视频

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter12:15

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

Published on: May 29, 2019

8.8K
Evaluation of Colorectal Cancer Risk and Prevalence by Stool DNA Integrity Detection07:35

Evaluation of Colorectal Cancer Risk and Prevalence by Stool DNA Integrity Detection

Published on: June 8, 2020

7.0K

相关概念视频

Base Excision Repair01:54

Base Excision Repair

One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template.
The first step of...
22.5K
Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
57.4K
Long-patch Base Excision Repair01:02

Long-patch Base Excision Repair

Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:
7.0K