非洲猪瘟病毒I329L基因的功能性特征通过转录组分析
Xianghan Duan1, Yuan Wen1, Panxue Wu1
1State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Veterinary microbiology
|August 14, 2023
概括
非洲猪瘟病毒 (ASFV) 基因I329L的功能被使用淘汰赛病毒探索. RNA-seq揭示ASFV I329L在感染期间影响宿主基因表达,帮助ASF控制策略.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 非洲猪瘟 (ASF) 是一种高度传染性的病毒性疾病,死亡率高.
- 非洲猪瘟病毒 (ASFV) 的基因组复杂性阻碍了疫苗开发.
- 了解ASFV基因功能对于疾病控制至关重要.
研究的目的:
- 为了研究ASFV I329L基因的功能.
- 分析ASFV I329L删除对宿主基因表达的影响.
- 为控制ASF提供有关ASFV与宿主相互作用的见解.
主要方法:
- 构建一个具有I329L基因删除的复合ASFV (ASFV CN/GS/2018-ΔI329L).
- 感染猪膜巨细胞 (PAMs) 的转录基因组测序 (RNA-seq).
- 对差异表达基因的基因本体学 (GO) 和KEGG通路分析.
- 使用RT-qPCR验证基因表达.
主要成果:
- 感染后12小时和24小时,ASFV I329L删除改变了宿主基因表达.
- 3和1基因的调节率上升,而12和19基因的调节率下降,分别为12和24hpi.
- RT-qPCR结果证实了对选定基因的RNA-seq发现.
结论:
- 在感染期间,ASFV I329L基因在调节宿主基因表达方面发挥作用.
- 这项研究为ASFV基因功能和宿主-病原体相互作用提供了新的见解.
- 这些发现有助于制定控制非洲猪瘟的战略.
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