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影响神经病理学的血液生物标志物的稳定性的预分析变量
Daniel Panikkar1, Sithara Vivek2, Eileen Crimmins3
1Department of Neuroscience, University of Minnesota, Minneapolis, MN, USA.
Journal of Alzheimer's disease : JAD
|August 14, 2023
概括
预分析条件,如处理延迟和冷解周期,会影响神经病理学的血液生物标志物. 标准化这些变量对于人口研究的可靠结果至关重要.
科学领域:
- 神经学 神经学
- 生物标志物发现发现
- 流行病学 流行病学
背景情况:
- 在大规模的神经病理学研究中,预分析协议显著影响血液生物标志物结果.
- 标准化样本采集对于数据完整性至关重要.
研究的目的:
- 在常见的预分析条件下,评估关键神经病理生物标志物的稳定性和检测变异性.
- 评估处理延迟和冷解周期对生物标志物测量的影响.
主要方法:
- 分析了41名参与者的血清和血样本.
- 在4°C,三个冷解周期和组合条件下,测试了长达72小时的处理延迟的影响.
- 使用Simoa测定方法测量了粉样蛋白-β 40 (Aβ40),粉样蛋白-β 42 (Aβ42),神经纤维光 (NfL),质纤维酸蛋白 (GFAP) 和酸化181 (p-tau-181).
主要成果:
- 随着处理延迟和冷解周期,Aβ40和Aβ42水平显著下降.
- 血清Aβ42/40比率在48小时内保持稳定,而血则略有增加.
- GFAP和NfL显示了温和的影响;血p-tau-181随着延迟而增加,但血清p-tau-181保持稳定.
结论:
- 像处理延迟和结解周期这样的分析前变量会影响神经病理生物标志物的稳定性.
- 标准化预分析条件对于人口研究中可靠和可重复的测量是必不可少的.
- 结果强调了在使用血液生物标志物的流行病学研究中需要仔细规范协议的需要.
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