在缺氧和骨质导电下,骨髓干细胞中的tRNA衍生小RNA变化
Chen Zhang1,2, Weilong Ye1, Mengyao Zhao1
1Laboratory of Molecular Signaling and Stem Cells Therapy, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Beijing Stomatological Hospital, School of Stomatology, Capital Medical University, Beijing, China.
Journal of oral rehabilitation
|August 14, 2023
概括
这项研究揭示了tRNA衍生小RNAs (tsRNAs) 调节骨髓干细胞 (BMSCs) 在缺氧下骨质分化. 这些tsRNAs在BMSC移植后提供了增强骨再生的潜在治疗点.
科学领域:
- 生物材料科学 生物材料科学
- 干细胞生物学 干细胞生物学
- 分子生物学分子生物学
背景情况:
- 组织工程用于骨再生利用骨介质干细胞 (BMSCs).
- 在缺氧下BMSC的功能异常限制了治疗疗效.
- 在低氧状态下研究BMSCs的非编码RNA调节至关重要.
研究的目的:
- 在低氧条件下探索BMSC骨质分化的机制.
- 为了确定参与调节BMSC对低氧反应的非编码RNA.
- 为了发现骨再生的潜在治疗点.
主要方法:
- 隔离了BMSC并使用CoCl2刺激对其进行过氧.
- 高通量测序确定了差异表达的tRNA衍生小RNA (tsRNAs).
- 生物信息分析预测了tsRNA目标基因和丰富的途径;实时RT-PCR验证表达.
主要成果:
- 在低氧状态下,在BMSC中分别表达了19个tsRNA (14个上调,5个下调).
- 这些tsRNAs可能调节311个参与代谢,Wnt信号和骨质细胞分化途径的基因.
- RT-PCR结果证实了所选的tsRNAs的表达模式.
结论:
- 在BMSC中,tsRNAs在骨质生成和缺氧反应中发挥着重要作用.
- 已识别的tsRNAs代表了改善BMSC生存和功能的新目标.
- 这项研究为增强骨再生疗法提供了新的途径.
相关概念视频
01:24Regulation of Angiogenesis and Blood Supply
2.6K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.6K
01:23Hypoxia
1.1K
Hypoxia is a medical condition characterized by an inadequate oxygen supply to body tissues. It typically manifests as a bluish discoloration of the skin and mucosae, especially in fair-skinned individuals, when hemoglobin (Hb) saturation drops below 75%.
Types of Hypoxia
There are four primary types of hypoxia, each resulting from a different cause:
1. Anemic hypoxia: This type occurs due to insufficient oxygen delivery caused by a lack of red blood cells (RBCs) or RBCs with abnormal or...
Types of Hypoxia
There are four primary types of hypoxia, each resulting from a different cause:
1. Anemic hypoxia: This type occurs due to insufficient oxygen delivery caused by a lack of red blood cells (RBCs) or RBCs with abnormal or...
1.1K
01:01Regulation of Hematopoietic Stem Cells
3.2K
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
3.2K