肌肉线粒体移植可以拯救和维持细胞平衡
Debasmita Bhattacharya1, Mikhaela B Slavin1, David A Hood1
1Muscle Health Research Center, School of Kinesiology and Health Science, York University, Toronto, Ontario, Canada.
American journal of physiology. Cell physiology
|August 14, 2023
概括
骨肌肉线粒体可以转移到其他细胞,改善它们的功能和能量生产. 这种线粒体转移会使细胞再生,并可能为疾病提供治疗策略.
科学领域:
- 细胞生物学 细胞生物学
- 线粒体生物学 线粒体生物学
- 再生医学是一种再生医学.
背景情况:
- 线粒体对于细胞能量生产和功能至关重要.
- 细胞之间的线粒体转移是最近的一个具有治疗潜力的研究领域.
- 骨肌肉线粒体在这个过程中的作用在很大程度上是未被探索的.
研究的目的:
- 研究骨肌肉线粒体转移到其他细胞类型的潜力.
- 为了确定转移的线粒体对宿主细胞功能和恒温的影响.
- 探索线粒体转移的机制及其治疗影响.
主要方法:
- 从小鼠骨肌中分离了介质纤维细胞线粒体.
- 隔离的线粒体与宿主肌细胞和人类纤维细胞的化.
- 分析线粒体的结合,网络的形成,以及生物能特征.
- 在抑制呼吸和 lysosomal 活动下评估细胞功能.
- 转移机制的研究,包括道化纳米管,细胞外囊泡,间隙结和巨细胞结.
- 在具有线粒体DNA突变的细胞中评估线粒体转移.
主要成果:
- 骨肌肉线粒体成功地以剂量和时间依赖的方式被纳入肌细胞.
- 线粒体转移增强了宿主细胞的生物能量,线粒体网络的延长和功能能力.
- 转移改善了细胞功能,即使在抑制呼吸和溶酶体活动的条件下.
- 线粒体通过多种机制转移,包括道纳米管和细胞外囊泡.
- 成功转移到具有线粒体DNA突变的人类纤维细胞,改善了新陈代谢功能和减少了活性氧物种 (ROS).
结论:
- 鼠骨肌线粒体可以有效地转移到健康和受损的宿主细胞.
- 线粒体转移完善线粒体结构,增强呼吸功能,增强细胞平衡.
- 这种线粒体贩运和生物能量重编程为各种疾病提供了潜在的治疗策略.
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