孕产妇表皮生长因子促进新生儿克劳丁-2依赖小肠透性的增加
Megan R Beggs1,2, Kennedi Young1, Allen Plain1
1Department of Physiology, University of Alberta, Edmonton, AB T6G 2H7, Canada.
Function (Oxford, England)
|August 14, 2023
概括
母乳通过克劳丁-2增强年轻动物的肠道吸收,克劳丁-2是骨发育至关重要的蛋白质. 牛奶中的表皮生长因子 (EGF) 推动了这一过程,其缺乏会损害骨矿化.
科学领域:
- 身体生理学 身体生理学
- 分子生物学分子生物学
- 发展生物学 发展生物学
背景情况:
- 与血液相比,母乳中的较高度促进了对细胞的吸收,以促进产后生长.
- 在哺乳动物中调节增强肠道吸收的分子机制尚未完全阐明.
研究的目的:
- 为了确定哺乳动物是否表现出肠道的透性增加,以最大限度地吸收.
- 为了确定潜在的分子机制,特别关注claudins,负责这种增强的透性.
主要方法:
- 检查了小鼠和Caco-2细胞在不同年龄段以及对荷尔蒙或母乳的反应中的肠道克劳丁 (CLDN) 表达.
- 测量了野生型,克劳丁-2 (Cldn2) 和克劳丁-12 (Cldn12) 淘汰赛小鼠以及Caco-2细胞的肠道透度.
- 在小鼠中使用微型计算机断层扫描 (μCT) 评估骨矿化.
主要成果:
- 与2个月大小的小鼠相比,2周大小的小鼠的肠道透率是2倍的.
- 克劳丁-2 (CLDN2) 表达在年轻小鼠中较高,而Cldn2淘汰的小鼠显示透率降低和骨矿化不足.
- 母乳中的表皮生长因子 (EGF) 特别增加了Caco-2细胞中的CLDN2表达和透度,而断奶则降低了CLDn2表达.
结论:
- 克劳丁-2调解了哺乳动物的肠道透率的增加,这对产后生长和骨发育至关重要.
- 母乳中的表皮生长因子 (EGF) 是克劳丁-2表达和肠道吸收的关键调节者.
- 破坏CLDN2通路导致在出生后早期发育过程中骨矿化受损.
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