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CsBZIP40通过抑制CsWRKY43-CsPrx53/CsSOD13级联介导的ROS清理,从而赋予了对类细菌癌症的抵抗力

Qiang Li^1,2,3, Xiujuan Qin¹, Miao Zhang¹

¹Citrus Research Institute, Southwest University/Chinese Academy of Agricultural Sciences, Beibei, Chongqing 400712, China.

Horticulture research

|August 14, 2023

概括

CsBZIP40基因通过抑制CsWRKY43.43,增强类细菌癌症 (CBC) 耐药性. 这一途径调节反应性氧物种 (ROS) 清理,为类植物的CBC预防提供了新的策略.

科学领域:

植物病理学植物病理学
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

类细菌性癌症 (CBC),是由Xanthomonas citri*亚种引起的. *citri* (*Xcc*),严重影响了全球果产量.
以前的研究表明,CsBZIP40增强了甜 (*Citrus sinensis*) 中的CBC耐药性,但根本机制尚不清楚.

研究的目的:

阐明CsBZIP40在甜中对CBC产生抗性的分子机制.
调查CsBZIP40在调节活性氧物种 (ROS) 恒温的作用及其与CBC耐药性的联系.

主要方法:

转基因甜植物产生过度表达CsBZIP40.
使用促进体结合试验 (TGA-盒,W-盒) 对基因表达的分析.
在转基因和淘汰/过度表达线路中评估CBC耐药性和ROS水平.

主要成果:

CsBZIP40过度表达增强了CBC耐药性,并调节了ROS平衡.
CsBZIP40直接抑制了 CsWRKY43 的转录.
CsWRKY43正调节抗氧化酶CsPrx53和CsSOD13,对ROS稳态和CBC耐药性产生负面影响.

结论:

一个新的调节途径被确定,其中CsBZIP40抑制了CsWRKY43-CsPrx53/CsSOD13级联,调节ROS清理CBC抵抗.
CsBZIP40,CsWRKY43,CsPrx53和CsSOD13代表了开发针对CBC的战略的潜在目标.

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