在番茄果实中进行的转录基因分析揭示了参与寒冷应激反应和果实成熟的基因的分歧
Oscar W Mitalo1, Seung Wong Kang1,2, Long T Tran1
1Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan.
Frontiers in plant science
|August 14, 2023
概括
冷藏会导致番茄的冷却损伤 (CI),但其机制尚不清楚. 这项研究表明,寒冷压力独特地改变了基因表达,与成熟不同,为改善水果耐寒性提供了洞察力.
科学领域:
- *植物生理学 植物生理学
- * 分子生物学 * 分子生物学
- * 收获后生物学
背景情况:
- *冷藏可以延长园艺作物的寿命,但会导致冷却损伤 (CI).
- *水果中冷应激和CI的机制尚不清楚,通常因成熟而混.
- *乙烯信号在水果成熟和对环境压力的反应中起作用.
研究的目的:
- * 为了研究1-甲基cyclopropene (1-MCP),一个乙烯抑制剂,对番茄果实成熟和CI的影响.
- *分析在寒冷压力下基因表达的变化,并确定特定于寒冷的反应.
- * 区分冷应激的分子路径与水果成熟的分子路径.
主要方法:
- * 成熟的绿色番茄被用1-MCP处理并存储在20°C或5°C.
- *评估了乙烯产量,颜色变化和CI症状.
- *RNA-Seq用于分析不同存储条件下的转录基因调整.
主要成果:
- *1-MCP在20°C时抑制成熟,但在5°C时对冷感应反应的影响有限.
- * 在5°C的冷藏 induced CI症状 (,衰变) 在重新加热时,不管是1-MCP.
- * 寒冷压力 (5°C) 引发了比成熟条件 (20°C,4814个基因) 更大的转录基因转移 (8,406个基因).
- * 感冒反应基因与成熟相关基因不同,涉及核糖体生物发生,ncRNA代谢,DNA甲基化和染色质重塑.
结论:
- * 寒冷压力会在番茄中引起独特的转录基因反应,与以乙烯介导的成熟分开.
- *1-MCP在长时间冷藏下对预防CI症状无效.
- *了解寒冷特异性基因表达对于开发提高水果耐寒性和减少收获后损失的策略至关重要.
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