微粒的高效惯性分离使用升高的柱状储和技术,用于 Lab-on-a-Chip 应用
Amir Mohamadsharifi1, Hassan Hajghassem1, Mahsa Kalantar1
1Faculty of New Sciences and Technologies, University of Tehran, Tehran 14759-87353, Iran.
ACS omega
|August 14, 2023
概括
这项研究使用重新设计的旋分离芯片改善了循环瘤细胞 (CTC) 隔离. 新设计提高了捕获CTC的效率和能力,这对于癌症诊断和预后至关重要.
科学领域:
- 生物医学工程 生物医学工程
- 微流体学 微流体学
- 癌症的诊断 癌症的诊断
背景情况:
- 循环瘤细胞 (CTC) 是癌症诊断和预后的重要生物标志物.
- 状分离是一种有前途的无标签方法,用于在芯片实验室系统中进行CTC隔离.
- 之前的形分离设计在效率和处理时间方面面临限制.
研究的目的:
- 提高基于的CTC隔离的效率和容量.
- 为了研究芯片几何学修改对粒子分离的影响.
- 开发一种改进的CTC捕获和分析方法.
主要方法:
- 重新设计芯片几何形状,使用升高的柱状储 (ECR) 来改善颗粒捕获.
- 使用数值模拟来分析第三轴对分离的贡献.
- 使用微粒 (20微米和8微米) 来表示CTC和较小的血细胞.
- 开发一个升级的注射系统和一个气泡净化方法.
主要成果:
- 微粒的平均分离效率达到80%±8.3%,增长了14%.
- 在微粒子分离中获得61%的纯度.
- 与以前的设计相比,它在保持轨道粒子的容量超过了前三倍.
- 成功描述了新的芯片几何形状,并验证了其性能.
结论:
- 经过重新设计的高度可变芯片与ECR显著提高了微粒分离效率和容量.
- 这种增强的旋分离方法为CTC隔离提供了更有效的解决方案.
- 开发的技术促进了用于癌症研究的微流体设备的稳健操作.
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