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现型诊断和基因型识别Bacillus cereus导致牛的亚临床乳腺炎

Rasha H Eid¹, Noha E Aref¹, Eman S Ibrahim²

¹Department of Mastitis and Neonatal Disease, Animal Reproduction Research Institute, Agriculture Research Center, Giza, Egypt.

Veterinary world

|August 14, 2023

概括

谷杆菌是埃及牛乳炎的常见原因,携带有关肠毒素和抗生素耐药性的基因. 这突出了与乳牛滥用抗生素相关的潜在公共卫生风险.

科学领域:

兽医微生物学兽医微生物学
食品安全食品安全
乳制品科学乳制品科学

背景情况:

牛乳炎对全球乳制品行业产生重大影响,其中*Bacillus*物种是常见的致病因子.
由 *Bacillus* spp. 引起的亚临床乳腺炎. 在乳牛群管理和牛奶质量方面带来了挑战.
了解埃及乳制品农场中*Bacillus*的流行率和特征对于疾病控制至关重要.

研究的目的:

在埃及,从患有亚临床乳腺炎的奶牛的生牛奶中分离和识别*Bacillus*物种.
为了确定分离的 *Bacillus* 菌株的抗生素敏感性模式.
检测在分离物中存在肠毒性和抗生素耐药性的基因.

主要方法:

对262个生牛奶样本进行了显微镜,生化和表型检查.
聚合酶链反应 (PCR) 用于基因型识别和基因检测.
阿加尔井扩散方法用于抗生素敏感性测试.

主要成果:

在47.7%的牛奶样本中发现了*Bacillus cereus*.
观察到肠毒素基因 (*Nhe*和 *hblD*) 和β-乳糖抗生素耐药基因的高患病率.
分离体对特定抗生素 (cefepime,cefixime,oxacillin) 呈现耐药性,但对其他抗生素 (阿莫西西林 - 克拉夫兰尼克,氨基醇,安比西林 / 苏尔巴克坦,莱沃弗洛克萨辛) 呈现敏感性.

结论:

强调B. cereus作为埃及乳牛患有乳腺炎的流行病原体.
强调由于抗生素耐药性和乳腺炎管理中的滥用而造成的潜在公共卫生威胁.
强调需要提高对该地区牛乳炎的认识和改善对抗策略.

关键词:

埃及埃及埃及埃及抗生素抗生素是一种抗生素.奶牛牛奶牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛哺乳期哺乳期哺乳期

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