不同表达的循环长非编码RNA在早产婴儿有呼吸困扰综合征的早产婴儿
1Department of Neonatology, Children's Hospital of Nanjing Medical University, Nanjing, Jiangsu 210008, P.R. China.
Balkan journal of medical genetics : BJMG
|August 14, 2023
概括
这项研究在患有呼吸困扰综合征 (RDS) 的婴儿血液中确定了特定的长非编码RNA (lncRNA). 这些lncRNAs可能在RDS的发展中发挥作用,为其机制提供了新的见解.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 新生儿医学 新生儿医学
背景情况:
- 长非编码RNAs (lncRNAs) 越来越多地被认为是它们在生物过程中的角色.
- 关于lncRNAs在新生儿呼吸困扰综合征 (RDS) 的参与存在有限的研究.
研究的目的:
- 研究和比较RDS婴儿与健康对照的循环lncRNAs的表达特征.
- 为了识别与RDS病变发生相关的特定lncRNAs.
主要方法:
- 从10名RDS婴儿和5名对照婴儿收集了血液样本.
- 进行了lncRNA表达概况,随后进行了生物信息学分析,以确定差异表达的lncRNAs.
- 量化逆转录PCR (qRT-PCR) 用于验证特定的lncRNA候选物.
主要成果:
- 在RDS和对照组之间确定了135个差异表达的lncRNAs.
- 四种特定的lncRNAs (ENST00000470527.1,ENST00000504497.1,ENST00000417781.5和ENST00000440408.5) 在RDS患者中表达增加,特别是在严重的情况下.
- 生物信息学分析表明,这些lncRNAs调节了PI3K-Akt,RAS,MAPK和TGF-β等关键信号通路.
结论:
- 特定的循环lncRNAs,包括ENST00000470527.1,ENST00000504497.1,ENST00000417781.5和ENST00000440408.5,与新生儿呼吸困扰综合征有关.
- 这些发现为早产RDS的潜在病理生理机制提供了新的见解.
- 已识别的lncRNAs可能作为RDS的潜在生物标志物.
相关概念视频
lncRNA - Long Non-coding RNAs
8.6K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.6K
RNA Splicing
56.5K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
56.5K
Alternative RNA Splicing
21.3K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
21.3K
Regulation of Expression at Multiple Steps
944
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
944
piRNA - Piwi-interacting RNAs
6.9K
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
6.9K
Translational Regulation
45
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
45


