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金属有机框架的微流体单步合成用于骨关节炎治疗的微RNA输送

Kaiyuan Yang¹, Min Ni², Chao Xu¹

¹Department of Orthopedics, The Affiliated Changzhou Second People's Hospital of Nanjing Medical University, Changzhou, China.

Frontiers in bioengineering and biotechnology

|August 14, 2023

概括

这项研究介绍了ZIF-8纳米颗粒,用于输送微RNA (miRNA),克服毒性和吸收问题. 这种新型的微流体方法增强了miRNA输送,用于潜在的骨关节炎治疗.

科学领域:

生物材料科学生物材料科学
纳米技术纳米技术
分子生物学分子生物学

背景情况:

微RNA (miRNA) 是简短的非编码RNA,调节基因表达,具有治疗潜力.
微RNAs的临床应用受到毒性,低产量,细胞吸收不足和内体细胞陷的阻碍.

研究的目的:

使用ZIF-8金属有机框架开发一种新型的低毒性miRNA传递载体.
增强细胞吸收和miRNAs的内体体逃逸,以改善核酸药物输送.
评估miR-200c-3p@ZIF-8在关节炎治疗中的疗效.

主要方法:

使用Y形微流体芯片制备miR-200c-3p@ZIF-8.
通过TEM,粒子大小分析,XRD,XPS和zeta潜力对miR-200c-3p@ZIF-8进行表征.
在实验室中评估ZIF-8载体的细胞毒性,miRNA加载效率,细胞吸收和内分体逃逸.

主要成果:

微流体方法产生了miR-200c-3p@ZIF-8,与水热方法相比,封装效率更高,颗粒大小更均.
ZIF-8载体表现出低细胞毒性和高miRNA加载效率.
观察到细胞吸收和miRNAs内体逃逸的显著改善.

结论:

ZIF-8纳米颗粒为核酸药物输送提供了强大而通用的战略,克服了关键的局限性.
准备好的miR-200c-3p@ZIF-8在治疗骨关节炎方面是有效的,展示了其治疗潜力.

关键词:

送货送货送货送货送货送货金属有机框架的框架.在 miR-200c-3p 中使用.微流体合成的微流体合成骨关节炎是一种关节炎.

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相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...