SwrA将DegU扩展到UP元素上,以激活Bacillus subtilis中的鞭毛基因表达
bioRxiv : the preprint server for biology
|August 14, 2023
概括
在 Bacillus subtilis 中,SwrA 和 DegU 形成一个复合体,以激活鞭毛基因表达. 这种异构体复合体结合了DNA,增强了RNA聚合酶与促进体和UP元素的相互作用,以提高运动性.
科学领域:
- 细菌转录调节 细菌转录调节
- 基因表达的分子机制
- 细胞运动性 细胞运动性
背景情况:
- 在Bacillus subtilis中,鞭毛基因表达对运动性和群体性至关重要.
- 转录启动涉及RNA聚合酶,促进剂,UP元素和转录因子.
- 在鞭毛基因调节中SwrA和DegU的作用尚未完全理解.
研究的目的:
- 研究SwrA和DegU调节Bacillus subtilis中鞭毛基因表达的机制.
- 阐明SwrA/DegU复合体的DNA结合特性和相互作用.
- 了解这个复合体是如何促进RNA聚合酶招募和转录激活的.
主要方法:
- 染色体免疫沉降测序 (ChIP-seq) 用于识别SwrA结合部位.
- 识别DegU特定的DNA序列.
- 分析SwrA对DegUDNA结合亲和力和足迹大小的影响.
- 与促进体相对的DegU结合部位位置的突变分析.
主要成果:
- SwrA与依赖于响应调节器DegU的众多染色体位点相互作用.
- 确定了一种DegU特定的反转重复DNA序列.
- SwrA 增强了 DegU DNA 的结合亲和力,并扩大了它对促进体的结合足迹.
- 靠近促进子的DegU结合部位的最佳定位对于转录激活至关重要.
结论:
- SwrA 和 DegU 形成一个异构复合体,激活 Bacillus subtilis 中的鞭毛基因表达.
- 这种复合物使得远程DNA结合和与RNA聚合酶和UP元素的相互作用成为可能.
- 这种异构体激活剂可能在细菌中常见,用于调节复杂的基因位点.
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