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Updated: Jul 19, 2025

Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting
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Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting

Published on: April 9, 2019

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通过使用 blotRig 分析软件,提高西方 blot 实验的严谨性和可重复性

Cleopa Omondi1, Austin Chou1, Kenneth A Fond1

  • 1Weill Institute for Neurosciences, University of California, San Francisco, San Francisco, CA USA.

bioRxiv : the preprint server for biology
|August 14, 2023
PubMed
概括

可复制的西方斑点实验需要严格的定量分析. 我们介绍了最佳实践和blotRig工具,以改善西部斑块数据分析和实验可重复性.

关键词:
分析化学 分析化学抗体是一种抗体.生物统计学 生物统计学计算生物学 计算生物学计算化学计算化学西部布洛特 (Western Blot) 是一个很大的问题.

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相关概念视频

Western Blotting01:15

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.
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