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对N-甘氨酸混合结构的选择性生物对等探针

Mana Mohan Mukherjee¹, Lara K Abramowitz¹, Bhoj Kumar²

¹Laboratory of Cell and Molecular Biology, NIDDK, National Institutes of Health, Bethesda, MD.

Research square

|August 14, 2023

概括

研究人员开发了一种新的化学报告员,专门标记混合N-甘氨酸,一种类型的糖蛋白. 这种工具可以精确地对细胞内的混合N-甘氨酸载体蛋白进行成像.

科学领域:

葡萄糖生物学葡萄糖生物学
化学生物学化学生物学
细胞成像细胞成像

背景情况:

使用标记单糖的糖蛋白和糖脂的代谢标记是具有挑战性的,因为共享的前体路径.
在标签上实现选择性是很困难的,特别是在复杂和无处不在的N-链 glycosylation,它有三个主要的 GlcNAc 含 N-glycan 结构 (寡甘,混合,和复杂).

研究的目的:

合成一种新的代谢化学报告器 (MCR) 用于混合N-甘氨酸结构的特定标记.
制定一个总体策略来定义化学标记单糖标签的选择性.
研究含有混合N-甘氨酸的蛋白质的细胞内定位和贩运.

主要方法:

1,3-Pr₂-6-OTs GlcNAlk作为下一代MCR的合成.
制定和应用一项策略,以确定标签选择性.
使用MCR进行细胞成像来追踪混合N-glycan携带蛋白质.

主要成果:

合成的MCR,1,3-Pr₂-6-OTs GlcNAlk,被证实是专门纳入混合N-glycans.
开发的战略成功地定义了新的MCR的选择性.
成像实验揭示了蛋白质与混合N-glycans的细胞内定位和贩运模式.

结论:

1,3-Pr2-6-OTs GlcNAlk代表了下一代的MCR用于有针对性的混合N-甘氨酸标签.
开发的方法提供了一个可靠的方法来评估MCR选择性.
这项工作使得混合N-甘氨酸相关蛋白质动态的高级成像研究成为可能.

关键词:

1,3-Pr2-6-OTs GlcNAlk 是一种的化合物.生物对等化学的化学.酶性标记是一种酶性标记.纤维拉林核蛋白是一种核蛋白.混合N-甘氨酸是一种混合N-甘氨酸.

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