巨细胞形成综合素介导的粘附环,以断表面结合的物体以进行细胞化
bioRxiv : the preprint server for biology
|August 14, 2023
概括
巨细胞使用新型的细胞粘附环 (PARs) 来吞与表面结合的物体. 这些结构利用来自actin聚合的integrin张力,而不是myosin II,以克服有效的细胞分裂的结合力.
科学领域:
- 细胞生物学 细胞生物学
- 免疫学 免疫学 免疫学
- 生物物理学的生物物理.
背景情况:
- 巨细胞是关键的免疫细胞,通过细胞分裂吞病原体和碎片.
- 悬浮颗粒的内部化已被很好地理解,但与表面结合的物体吸收仍然不清楚.
研究的目的:
- 阐明巨细胞内部化表面绑定微米大小的物体的机制.
- 研究细胞力和粘附结构在克服表面结合中的作用.
主要方法:
- 开发一种力感应平台,可可视化细胞基质粘合力.
- 在巨细胞中与固定物体 (大肠杆菌,微珠,银纳米棒) 相互作用的整合素传递力和结构蛋白的联合成像.
主要成果:
- 巨细胞在表面的物体周围形成"食细胞粘附环" (PAR).
- 在 PAR 中的整合蛋白张力是由局部的活性蛋白聚合生成的,独立于肌肉蛋白II.
- PAR整基因张力强度与物体结合强度相关,并决定了细胞化效率.
结论:
- 巨细胞使用PARs作为一种用于细胞化表面结合材料的新机制.
- PARs为actin聚合提供了定点,使得巨细胞能够提升和内化附着的物体.
- 整合素连接体强度是表面绑定的细胞效率的关键决定因素.
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