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通过一种新的基于铜的3,4-dimethoxycinnamic 酸复合物来抑制人类酶聚合,这种复合物由3,4-dimethoxycinnamic 酸组成

Sumra Dilshad¹, Shabnam², Arif Ali¹

¹Department of Applied Chemistry, ZHCET, Aligarh Muslim University, Aligarh, India.

Journal of biomolecular structure & dynamics

|August 14, 2023

概括

一个新的铜 (II) 复合体,标记为1,有效地抑制了人体酶 (HuL) 纤维化. 这种化疗剂适度地与HuL结合,防止粉样蛋白的形成并保持蛋白质结构.

科学领域:

协调化学协调化学
生物化学生化学
材料科学材料科学材料科学

背景情况:

蛋白质纤维化,特别是在人类酶 (HuL) 中,与各种疾病有关.
开发小分子来抑制蛋白质聚合是关键的治疗策略.

研究的目的:

合成和表征一种新的基于铜的复合物,[Cu(DCA) 4 ((H2O) 2·4H2O·4MeOH (1),其中DCA是3,4-二度氧化酸.
调查复合1在抑制HuL纤维化的有效性.
阐明复合体1和HuL之间的相互作用机制.

主要方法:

使用单晶X射线衍射 (SC-XRD),UV-Vis,FTIR,PXRD和TGA.复合铜的合成和表征.
光定位,对接和模拟研究,以评估与HuL的结合相互作用.
提奥夫拉T (ThT) 试验,光散射,ANS疏水性试验,CD光谱和原子力显微镜 (AFM) 来评估纤维化抑制.

主要成果:

复合物1成功合成和表征,揭示了带有扭曲方形金字塔式Cu (II) 离子的轮结构.
复合物1在pH 2时对HuL (Ka = 6.3x10^5 M^-1) 呈现适度的结合,与特定的氨基酸残留相互作用.
复合物1以度依赖的方式抑制了HuL纤维化,防止了疏水性,富含β片的粉样纤维的形成,并促进了更小,更少疏水性的聚合物,保留了α螺旋结构.

结论:

合成的铜 (II) 复合体1是一种有效的抑制剂,可以抑制人类的酶纤维化.
该机制涉及对蛋白质的中度结合,导致非粉样聚合物的形成.
复合物1显示出作为治疗蛋白质错折疾病的化疗剂的潜力.

关键词:

复杂的铜复合体铜复合体聚合预防防止聚合氨基粉症是什么?动是因为纤维化.

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