工程库马林积累减少了菌毒素诱导的氧化应激和疾病易感性
Alexander Beesley1, Sebastian F Beyer1, Verena Wanders1
1Department of Plant Physiology, RWTH Aachen University, Aachen, Germany.
Plant biotechnology journal
|August 14, 2023
概括
在作物中的工程氨酸生产增强了对疾病的抵抗力. 过度表达一个关键的酶提高了库马林水平,降低了对植物线虫和真菌病原体的敏感性.
科学领域:
- 植物科学 植物科学
- 生物技术是生物技术.
- 农业科学 农业科学
背景情况:
- 氨酸表现出抗微生物特性,显示出对抗病原体作物保护的潜力.
- 在农业生物技术中,在作物中的工程氨酸生物合成仍然是一个重大挑战.
研究的目的:
- 在作物植物和模型系统中设计库马林的构成性积累.
- 评估增强库马林水平对植物对特定害虫和病原体的防御的影响.
主要方法:
- 在 *Nicotiana benthamiana*, *Glycine max* 和 *Arabidopsis thaliana* 中过度表达了 Arabidopsis thaliana 铁-CoA 6 - 基酶 1 (AtF6'H1).
- 对转基因植物和细胞培养中的库马林配置的分析.
- 评估植物对 *Heterodera schachtii* 线虫和 *Fusarium virguliforme* 菌的耐药性.
主要成果:
- 转基因植物和细胞表现出 scopoletin 和 scopolin 的构成性积累.
- 机械损伤将斯科波林从斯科波林池中释放出来.
- 在*A. thaliana*根中的高斯科波林水平与线虫敏感性降低相关.
- 转基因大豆植物对*Fusarium virguliforme*的耐受性增加.
结论:
- 工程库马林积累增强了植物对生物压力的弹性.
- 过度表达AtF6'H1是一种可行的策略,可以改善作物抗病能力.
- 减少的氧化损伤有助于增强库马林过度积累植物的病原体耐受性.
关键词:
库马林是一种素.疾病耐药性 疾病耐药性菌类毒素 (mycotoxin) 是一种有毒的物质.有活性氧物种的反应性氧物种.豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆压力耐受性 耐压力耐受性更多相关视频
09:33Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors
Published on: February 7, 2018
7.5K
07:40Protection of H9c2 Myocardial Cells from Oxidative Stress by Crocetin via PINK1/Parkin Pathway-Mediated Mitophagy
Published on: May 26, 2023
1.2K
相关概念视频
Anticoagulant Drugs: Low-Molecular-Weight Heparins
753
Hemostasis is a crucial process that prevents excessive blood loss from damaged blood vessels. It involves various mechanisms such as vasoconstriction, platelet adhesion and activation, and fibrin formation. The importance of each mechanism depends on the type of vessel injury. In contrast, thrombosis is the abnormal formation of a blood clot within the blood vessels, leading to potential complications if the clot obstructs blood flow. Thrombosis can be caused by increased coagulability of the...
753
Anticoagulant Drugs: Vitamin K Antagonists and Direct Oral Anticoagulants
1.2K
Oral anticoagulants are vital tools in preventing and treating blood clotting disorders. This diverse class of medications can be categorized as vitamin K antagonists, exemplified by warfarin, and direct thrombin inhibitors (DTIs), such as dabigatran, as well as factor Xa inhibitors, including rivaroxaban.
Warfarin, a prominent vitamin K antagonist family member, exerts its effect by inhibiting the enzyme VKORC1 (vitamin K epoxide reductase complex 1). By hindering this enzyme, warfarin...
Warfarin, a prominent vitamin K antagonist family member, exerts its effect by inhibiting the enzyme VKORC1 (vitamin K epoxide reductase complex 1). By hindering this enzyme, warfarin...
1.2K
Electron Transport Chain: Complex I and II
14.5K
The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
ROS generation is regulated and maintained at moderate levels necessary...
14.5K
The Electron Transport Chain
16.9K
The electron transport chain or oxidative phosphorylation is an exothermic process in which free energy released during electron transfer reactions is coupled to ATP synthesis. This process is a significant source of energy in aerobic cells, and therefore inhibitors of the electron transport chain can be detrimental to the cell's metabolic processes.
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q...
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q...
16.9K
