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针对结核病疫苗的有效性评估的微殖民地形成单位试验

David Pires¹, Paulo J G Bettencourt²

¹Faculty of Medicine, Universidade Católica Portuguesa; Center for Interdisciplinary Research in Health, Universidade Católica Portuguesa; Host-Pathogen Interactions Unit, Research Institute for Medicines, iMed-ULisboa, Faculty of Pharmacy, Universidade de Lisboa.

Journal of visualized experiments : JoVE

|August 14, 2023

概括

一种新的微殖民地形成单位 (mCFU) 测定方法显著加快了结核病疫苗的研发速度. 这种方法可以减少时间,成本和实验室空间,相比于传统的殖民地形成单位 (CFU) 测定用于Mycobacterium结核病研究.

科学领域:

微生物学微生物学
疫苗学疫苗学疫苗学
传染性疾病传染性疾病

背景情况:

结核病 (TB) 仍然是全球主要的传染性死亡原因.
结核病疫苗在临床前研究中的有效性评估目前的标准是殖民地形成单位 (CFU) 试验.
传统的CFU测定耗时 (4-6周),资源密集,并带有污染风险.

研究的目的:

引入一种优化,快速的方法来清算Mycobacterium结核病 (M.tb) 的殖民地.
提出一种改进的方法来准备用于感染研究的菌根菌种.
倡导这些优化方法的普遍采用,以加速结核病疫苗的开发.

主要方法:

开发和验证微殖民地形成单位 (mCFU) 测定对M.tb.的测定.
优化单细胞,均,高质量的菌根菌培养生产.
在临床前模型中应用mCFU试验来分析结核病疫苗的疗效.

主要成果:

mCFU试验使试剂使用量减少了十倍,化时间减少了三倍 (1-2周).
mCFU测定尽量减少实验室空间需求和试剂成本.
优化培养制备可产生高质量,均的真菌菌样本用于感染研究.

结论:

在结核病疫苗研究中,mCFU试验为传统的CFU试验提供了更快,更有效,更安全的替代方案.
优化的菌根菌培养方法提高了临床前感染研究的可靠性.
采用这些简化方法可以显著加速开发新的结核病疫苗.

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