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基于文化学和测序分析的毛囊液微生物群的表征

Wenting Wei^1,2, Yazhou Zhou¹, Haiyang Zuo³

¹State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, PR China.

Journal of medical microbiology

|August 14, 2023

概括

卵泡液对于卵子发育至关重要,它不是无菌的,并且含有微生物. 这些微生物可以预测体外受精成功率,为生育治疗提供新的见解.

科学领域:

生殖生物学和微生物学.
研究人类卵细胞微环境及其微生物居民.

背景情况:

毛囊液对于卵细胞生长,排卵和成熟至关重要.
卵泡液中微生物的存在可能与体外受精 (IVF) 结果相关.
一些在卵泡液中存在微生物殖民的女性仍然没有症状.

研究的目的:

为了确认人类毛囊液中存在微生物的存在.
来自接受试管婴儿的妇女的卵泡液中的微生物特征.
为了将微生物存在与不同的试管婴儿结果相关联.

主要方法:

从接受试管婴儿的163名女性收集了毛囊液样本.
用16S rDNA测序进行微生物检测,分析了157个样本.
19个样本接受了微生物分离和识别的培养组.

主要成果:

文化学发现毛囊液样本不是无菌的,对*Streptococcus*,*Finegoldia*和*Peptoniphilus*的隔离率很高.
在所有157个样本中,16S rDNA测序检测到微生物,其中常见的是*Pseudomonas*,*Lactobacillus*,*Comamonas*,*Streptococcus*和*Acinetobacter*.
不同的细菌丰富度与怀孕率,受精率,胚胎质量和卵细胞产量相关.

结论:

人的卵泡液是一种非无菌的环境,包含各种各样的微生物.
毛囊液的微生物组成是体外受精结果的潜在预测因素.
对卵泡液微生物组的进一步研究可以提高试管婴儿的成功率.

关键词:

文化经济学文化经济学生育能力生育能力生育能力生育能力毛囊液体毛囊液体毛囊液体在体外受精体外受精.我们的微生物群.

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