来自奶牛犊的抗菌素耐药性大肠杆菌的表征
Merning Mwenifumbo1,2, Adrian L Cookson1,3, Shengguo Zhao4
1School of Veterinary Science, Hopkirk Research Institute, cnr University & Library Rds Massey University, Palmerston North 4442, New Zealand.
Journal of medical microbiology
|August 14, 2023
概括
抗多种药物的大肠杆菌在奶牛中很常见,特别是那些对四环素和 estreptomycin 耐药的. 需要进一步研究以了解农场管理与这些耐药大肠杆菌菌株之间的联系.
科学领域:
- 兽医微生物学 兽医微生物学
- 抗微生物耐药性 抗微生物耐药性
- 细菌基因组学 细菌基因组学
背景情况:
- 预先断奶的乳牛是多药耐药性 (MDR) Escherichia coli 的重要储库.
- 对于来自乳牛的大肠杆菌菌株及其耐药基因的遗传位置存在有限的表征.
研究的目的:
- 描述使用废牛奶的农场小牛中抗菌素耐药的大肠杆菌的表型和基因型特征.
- 为了确定这些耐药大肠杆菌菌株的种群结构.
- 为了研究抗微生物耐药性基因 (等离子体或染色体) 的位置.
主要方法:
- 来自40只年轻奶牛的直 swab 被分析了对四环素,链杆菌素,西普洛素和第三代素的耐药性.
- 整个基因组测序 (短读和长读) 在选择的抗菌素耐药性大肠杆菌分离物上进行.
- 牛津纳米孔长读测序确定了塑体上耐药性基因盒的位置.
主要成果:
- 在58%的小牛中发现了抗微生物耐药的大肠杆菌,其中高比例的抗四环素 (43%) 和产生AMPC的大肠杆菌 (23%).
- 全基因组测序确定了五种大肠杆菌序列类型 (ST),其中ST88占主导地位 (63%),与其他肠外致病性大肠杆菌血统一起.
- 16个分离物是MDR,耐药基因位于IncF等离子体上,通过长读测序确定.
结论:
- 奶牛牛犊表现出对四环素和 estreptomycin 耐药性大肠杆菌的高患病率.
- 该研究确定了MDR大肠杆菌菌株,强调需要进一步调查农场管理实践.
- 了解耐药性基因位置对于开发有效的控制策略至关重要.
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