对于SARNP和DDX39B的高阶复合体的结构基础,以促进mRNP组装
Yihu Xie1, Shengyan Gao2, Ke Zhang2
1Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232-0146, USA; Center for Structural Biology, Vanderbilt University School of Medicine, Nashville, TN 37232-0146, USA.
Cell reports
|August 14, 2023
概括
这项研究揭示了SARNP/DDX39B复合体对于mRNA输出至关重要,它是如何形成一个高阶结构的. 这种复杂的,涉及双联动因子和RNA,是保存和影响GC丰富的RNA出口.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 结构生物学 结构生物学
背景情况:
- 从核到细胞质的mRNA输出对基因表达至关重要.
- 包括DDX39B在内的转录-出口 (TREX) 综合体介导mRNA包装和出口.
- SARNP (也称为Tho1) 与DDX39B相互作用并影响mRNA输出,但其机制尚不清楚.
研究的目的:
- 阐明DDX39B对SARNP识别的分子机制及其在信使核糖蛋白粒子 (mRNP) 组装中的作用.
- 为了确定TREX复合体内的SARNP-DDX39B相互作用的结构基础.
主要方法:
- 确定Tho1/DDX39B/RNA复合物的晶体结构.
- 分析SARNP和DDX39B之间的高阶复合体形成.
- 细胞的RNA测序 (RNA-seq) 分析与SARNP敲击.
主要成果:
- 晶体结构揭示了SARNP/Tho1和DDX39B之间的多价值相互作用,由并联DDX39B相互作用图案介导.
- SARNP/DDX39B形成了一个保存的高阶复合体,人类的SARNP能够结合多个DDX39B分子.
- RNA-seq数据表明,在SARNP淘汰后,GC丰富的RNA在出口中受到影响最大.
结论:
- 这些发现表明,mRNP组装和出口的模型涉及高级SARNP/DDX39B/RNA复合体.
- 这种对TREX复合体的结构和功能洞察力突出了SARNP在mRNA出口途径中的保留作用.
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