基于染色体供体的近红外电压敏感染料
Ping Yan1, Corey D Acker1, Valentina Biasci2
1Richard D. Berlin Center for Cell Analysis and Modeling, University of Connecticut Health Center, Farmington, CT 06030.
概括
新的电压敏感染料 (VSDs) 使用的是,而不是,提供红移光谱,以更清晰地光学成像细胞电活动. 这些染料可以精确监测刺激性组织中的作用潜力.
科学领域:
- 生物物理学的生物物理.
- 神经科学是一个神经科学.
- 光学成像技术的成像
背景情况:
- 电压敏感染料 (VSD) 对于细胞电活动的高速光学成像至关重要.
- 现有的VSD通常使用推拉色谱,限制光谱范围,并可能干扰光遗传工具.
- 红移染料是为了更深的组织透和减少光毒性而需要的.
研究的目的:
- 开发具有红移光谱的新型电压敏感染料.
- 为了评估这些新染料在成像心脏电活动中的性能.
- 评估这些染料与光遗传学操纵的兼容性.
主要方法:
- 合成基于染色体的染色体作为VSDs.
- 吸收和发射性质的光谱表征.
- 在小鼠心脏上进行体外和体外测试,包括同时进行光学成像和光遗传刺激.
主要成果:
- 替代导致光谱在60-80纳米的红色偏移,与纳夫他林基染料相比.
- 使用730nm激发,在ex vivo小鼠心脏中达到12%的ΔF/F每次作用电位.
- 在道罗多普辛表达心脏中证明了无人工物,全光学监测动作潜力的传播.
结论:
- 基于的VSD为电压成像提供了卓越的光谱特性.
- 这些染料可实现高分辨率,实时对心脏动作潜力的成像.
- 开发的VSD与光遗传学兼容,并有望在神经科学和生物物理学中得到更广泛的应用.
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