相关实验视频

Updated: Jul 19, 2025

Polarization and Characterization of M1 and M2 Human Monocyte-Derived Macrophages on Implant Surfaces

Polarization and Characterization of M1 and M2 Human Monocyte-Derived Macrophages on Implant Surfaces

Published on: December 6, 2024

光生物调节增强了桃体衍生的中细胞干细胞的M2巨分极特性

Ken Woo¹, So Young Park², Andrew Padalhin²

¹Gyeongnam International Foreign School, Sacheon, Republic of Korea.

Journal of photochemistry and photobiology. B, Biology

|August 14, 2023

概括

使用红色LED光疗法进行光生物调制 (PBM) 可以增强桃体衍生的介质干细胞 (TMSCs). 这种PBM初始化促进了M2巨细胞的两极分化,改善了诸如口腔粘膜炎和伤口愈合等疾病的治疗潜力.

科学领域:

再生医学是一种再生医学.
生物光子学生物光子学
免疫调节是一种免疫调节.

背景情况:

介质细胞干细胞 (MSCs) 显示出治疗前景,但需要优化.
桃体衍生的介质干细胞 (TMSCs) 是再生应用的可行来源.
调节免疫微环境对于提高MSC疗效至关重要.

研究的目的:

研究光生物调制 (PBM) 对TMSCs的影响.
评估PBM在调节巨细胞极化中的作用.
为了评估PBM-primed的TMSC,以提高治疗潜力.

主要方法:

用630nm的LED阵列对TMSC进行处理,能量剂量不同 (0,15,30,60 J).
用PBM治疗的TMSCs与M1-激活的RAW 264.7巨细胞共同培养.
分析了巨细胞极化标记物 (iNOS,Arginase-1) 和IL-1RA的基因表达.

主要成果:

在30 J的PBM治疗显著降低了M1 (iNOS) 和增加了M2 (阿基纳-1) 巨体标志物.
观察到抗炎细胞因子IL-1RA基因表达的显著增加.
与巨细胞共同培养的未经治疗的TMSCs没有显著的抗炎作用.

结论:

对TMSCs的PBM化有效地促进了M2巨细胞的两极分化.
PBM增强了TMSCs的免疫调节能力.
用PBM治疗的TMSC具有治疗口腔粘膜炎和促进伤口愈合的潜力.

关键词:

炎症炎症是一种炎症.巨细胞是一个巨细胞.巨细胞的两极分化摄影生物调制 (Photobiomodulation) 是一种生物调制方法.干细胞是一种干细胞.

更多相关视频

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

Published on: June 23, 2013

Mesenchymal Stem Cell Regulation of Macrophage Phagocytosis; Quantitation and Imaging

Mesenchymal Stem Cell Regulation of Macrophage Phagocytosis; Quantitation and Imaging

Published on: July 16, 2021

相关概念视频

Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...