在缓解Capsicum annuum中毒性的生理和分子机制
Chuang Shen1, Huiling Fu1, Baifei Huang1
1Research Center for Environmental Pollution Control Technology, School of Chemical and Environmental Engineering, Hunan Institute of Technology, Hengyang 421002, China.
The Science of the total environment
|August 14, 2023
概括
的应用改善了叶的生长和在压力下进行光合作用. 它增强细胞壁中的保留,增强抗氧化防御,最终减少积累.
科学领域:
- 植物生理学 植物生理学
- 环境科学 环境科学
- 生物化学 生化学
背景情况:
- 土壤 (Cd) 污染对食品安全和健康构成风险.
- (B) 可能会减少植物根中的Cd吸收,但其在Cd应激下叶子中的作用不太清楚.
- 研究B对基因表达,代谢物,叶子生长和叶中Cd积累的影响至关重要.
研究的目的:
- 研究B对叶生长和在Cd压力下Cd积累的影响.
- 为了确定B如何影响热胡叶中的基因表达和生理参数.
- 阐明B减轻植物中Cd毒性的机制.
主要方法:
- (B) 在 (Cd) 压力下的 (B) 应用于热植物.
- 测量包括叶绿素,二氧化碳度,口腔导电,光合作用和透气率在内的生理参数.
- 转录组测序以分析与细胞壁组件,抗氧化酶和金属载体相关的基因表达.
- 对pectin甲基化酶活性的测定和Cd在细胞壁中的保留量的量化.
主要成果:
- B应用显著改善了Cd压力中的光合作用率 (18-41%) 和含量.
- 通过调高与pectin,lignin和callose相关的基因和增加pectin甲基化酶活性 (30%),B增强了细胞壁中的Cd保留率,导致Cd保留率提高了~31%.
- B增强了抗氧化酶活动 (28-40%) 和参与硫代谢,HIPP和金属载体 (CAX3,YSL,ABC,ZIP,DTX) 的基因的上调,提高了Cd的耐受性.
结论:
- 的应用减轻了Cd对叶生长和光合作用的负面影响.
- B促进了Cd的分离和保留在细胞壁中,减少了其转移到果实中.
- 的应用增强了植物对Cd诱导的氧化应激的防御机制,并加强了整体的Cd耐受性.
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