通过适应性实验室进化获得的高阿斯塔丁产生的Phaffia rhodozyma/Xanthophyllomyces dendrorhous的转录学分析和料批次调节
Liang Yang1,2, Hao-Yi Yang1,2, Li You1,2
1College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China.
Journal of industrial microbiology & biotechnology
|August 14, 2023
概括
使用二氧化 (TiO2) 的适应性实验室进化成功增强了 Phaffia rhodozyma 中的阿斯塔山丁生产. 优化的菌株和源调节显著增加了功能性食品应用中的阿斯塔山丁产量.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 生物化学 生物化学
背景情况:
- 阿斯塔丁是一种强大的抗氧化剂,在功能性食品中具有高价值.
- 法菲亚·罗多齐玛 (Phaffia rhodozyma) 自然产生阿斯塔克桑,但产量通常较低.
- 提高阿斯塔克桑产量对于工业应用至关重要.
研究的目的:
- 使用适应性实验室进化来增强Phaffia rhodozyma中的阿斯塔桑生产.
- 为了研究导致阿斯塔克桑产量增加的代谢机制.
- 为了优化发酵条件以最大限度地提高阿斯塔丁含量.
主要方法:
- 使用二氧化 (TiO2) 作为氧化应激诱导剂的Phaffia rhodozyma的适应性实验室进化 (ALE).
- 在批量和料批量条件下发育菌株 (JMU-ALE105) 的发酵.
- 转录组分析以确定上调代谢途径.
- 在料批发发酵过程中优化源 (硫酸).
主要成果:
- 一种产量高的阿斯塔桑生产菌株JMU-ALE105在经过105天的TiO2化后被开发出来.
- 与原始菌株相比,ALE105菌株的阿斯丁含量增加了41.61%.
- 转录组学揭示了阿斯塔山丁合成,脂肪酸,酸和代谢途径的显著上调.
- 用调整的源进行料批发发酵,进一步增加了阿斯塔山丁含量,达到8.36 mg/g.
结论:
- 使用TiO2的适应性实验室进化对于开发P. rhodozyma. 的高产阿斯丁菌株是有效的.
- 了解代谢途径调节为进一步改进菌株提供了基础.
- 优化源料批发发酵显著提高了阿斯塔丁的生产,为工业化铺平了道路.
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