一个集成的有机体奥米克斯地图扩展了病的建模潜力
Moritz Lassé1,2, Jamal El Saghir3, Celine C Berthier3
1III. Department of Medicine, University Medical Center Hamburg-Eppendorf (UKE), Hamburg, Germany.
Nature communications
|August 14, 2023
概括
脏器官提供了对脏疾病的洞察力. 这项研究绘制了它们的蛋白质和基因表达变化的地图,揭示了病研究中新生物标志物发现的潜力.
科学领域:
- 生物技术是生物技术.
- 基因组学就是基因组学.
- 蛋白质组学是指蛋白质组学.
背景情况:
- 器官是研究病的有价值模型.
- 了解它们的功能性蛋白质表达是至关重要的,但有限.
- 对有机体蛋白质组和转录组的有限知识阻碍了疾病建模.
研究的目的:
- 定义脏器官蛋白质和转录蛋白质随时间的轨迹.
- 为了研究对瘤亡因子-α (TNFα) 暴露的有机体反应.
- 整合多组数据并与人类病数据进行比较.
主要方法:
- 在不同培养时间内对脏器官的蛋白质组和转录组分析.
- 单细胞转录组分析以定位蛋白质组变化.
- TNFα刺激测定和差异性蛋白质表达分析.
- 将有机体数据与蛋白尿性病患者的临床数据进行比较.
主要成果:
- 衰老的有机体显示出细胞外基质沉积的增加和质蛋白质表达的减少.
- 主要观察到的蛋白质组变化主要发生在细胞,管状细胞和层状细胞中.
- TNFα治疗诱导了322种差异表达的蛋白质,包括细胞因子和补充成分.
- 在有机体中TNFα相关蛋白质 (C3,VCAM1) 的表达反映了人类细胞中的发现.
结论:
- 器官精确地反映了人类病中与年龄相关的和压力引起的变化.
- 在有机体中TNFα诱导的蛋白质表达与蛋白尿性病的不良临床结果相关.
- 器官对促进病生物标志物开发和研究具有重大潜力.
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