树杆菌 (Rhizopus stolonifer) 生物质的催化转化能力:优化种植条件
Nadeem I Elhussieny1,2,3, Heba A El-Refai4, Sayeda S Mohamed4
1Department of Life Science and Chemistry, Constructor University, Campus Ring 1, 28759, Bremen, Germany. nelhussieny@constructor.university.
Microbial cell factories
|August 14, 2023
概括
为Rhizopus stolonifer优化真菌生物量培养,可以增强其生物催化活性. 本研究确定了改善废油油 (WFO) 转化的关键因素,从而提高脂肪酸甲基 (FAME) 的产量.
科学领域:
- 生物技术是生物技术.
- 工业微生物学 工业微生物学
- 生物催化剂是一种生物催化剂.
背景情况:
- 对于生物催化剂,酶成本可能是无法承受的.
- 菌生物质为生物催化剂提供了具有成本效益的替代品.
- 优化真菌种植对于有效的生物质活动至关重要.
研究的目的:
- 为了优化Rhizopus stolonifer生物质的种植条件.
- 为了提高R. stolonifer的转和水解能力,用于废炸油 (WFO) 的转化.
- 为了改善脂肪酸甲基 (FAME) 的生产.
主要方法:
- 对种植参数进行选,包括动条件,诱导剂,源,pH,鱼粉和KH2PO4.
- 利用普莱克特·伯曼的设计来进行因子选.
- 使用响应表面方法 (RSM) 与中央复合设计 (CCD) 进行优化.
- 评估生物质增长,脂酶活性和转化能力.
主要成果:
- 在200rpm的震动条件下,橄油作为诱导剂显著改善了生长和脂解活性.
- 与玉米和尿素相比,鱼粉是一种优越的源.
- 通过RSM确定了最佳条件 (pH7.4,2.62%的鱼粉,2.99%的KH2PO4).
- 确定的重要因素是pH,鱼粉和KH2PO4.
结论:
- 优化的培养条件显著促进了R. stolonifer生物质的全细胞转化.
- 脂肪酸甲基 (FAME) 度增加了67.65%. 脂肪酸甲基 (FAME) 度增加了67.65%.
- 达到了最终的FAME度为85.5% (w/w),证明了高效的WFO转换.
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